Redox regulation of SurR by protein disulfide oxidoreductase in Thermococcus onnurineus NA1 SCIE SCOPUS

Cited 4 time in WEB OF SCIENCE Cited 4 time in Scopus
Title
Redox regulation of SurR by protein disulfide oxidoreductase in Thermococcus onnurineus NA1
Author(s)
Lim, Jae Kyu; Jung, Hae-Chang; Kang, Sung Gyun; Lee, Hyun Sook
KIOST Author(s)
Jung, Hae Chang(정해창)Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)
Publication Year
2017-05
Abstract
Protein disulfide oxidoreductases are redox enzymes that catalyze thiol-disulfide exchange reactions. These enzymes include thioredoxins, glutaredoxins, protein disulfide isomerases, disulfide bond formation A (DsbA) proteins, and Pyrococcus furiosus protein disulfide oxidoreductase (PfPDO) homologues. In the genome of a hyperthermophilic archaeon, Thermococcus onnurineus NA1, the genes encoding one PfPDO homologue (TON_0319, Pdo) and three more thioredoxin- or glutaredoxin-like proteins (TON_0470, TON_0472, TON_0834) were identified. All except TON_0470 were recombinantly expressed and purified. Three purified proteins were reduced by a thioredoxin reductase (TrxR), indicating that each protein can form redox complex with TrxR. SurR, a transcription factor involved in the sulfur response, was tested for a protein target of a TrxR-redoxin system and only Pdo was identified to be capable of catalyzing the reduction of SurR. Electromobility shift assay demonstrated that SurR reduced by the TrxR-Pdo system could bind to the DNA probe with the SurR-binding motif, GTTttgAAC. In this study, we present the TrxR-Pdo couple as a redox-regulator for SurR in T. onnurineus NA1.
ISSN
1431-0651
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1246
DOI
10.1007/s00792-017-0919-1
Bibliographic Citation
EXTREMOPHILES, v.21, no.3, pp.491 - 498, 2017
Publisher
SPRINGER JAPAN KK
Keywords
Protein disulfide oxidoreductase; Thioredoxin reductase; SurR; Thermococcus onnurineus NA1; Redox system
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER JAPAN KK
Related Researcher
Research Interests

Microbial biotechnology,Marine enzymes,미생물 활용,효소 개발

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