Redox regulation of SurR by protein disulfide oxidoreductase in Thermococcus onnurineus NA1 SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Lim, Jae Kyu | - |
dc.contributor.author | Jung, Hae-Chang | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.date.accessioned | 2020-04-16T10:40:12Z | - |
dc.date.available | 2020-04-16T10:40:12Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 1431-0651 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/1246 | - |
dc.description.abstract | Protein disulfide oxidoreductases are redox enzymes that catalyze thiol-disulfide exchange reactions. These enzymes include thioredoxins, glutaredoxins, protein disulfide isomerases, disulfide bond formation A (DsbA) proteins, and Pyrococcus furiosus protein disulfide oxidoreductase (PfPDO) homologues. In the genome of a hyperthermophilic archaeon, Thermococcus onnurineus NA1, the genes encoding one PfPDO homologue (TON_0319, Pdo) and three more thioredoxin- or glutaredoxin-like proteins (TON_0470, TON_0472, TON_0834) were identified. All except TON_0470 were recombinantly expressed and purified. Three purified proteins were reduced by a thioredoxin reductase (TrxR), indicating that each protein can form redox complex with TrxR. SurR, a transcription factor involved in the sulfur response, was tested for a protein target of a TrxR-redoxin system and only Pdo was identified to be capable of catalyzing the reduction of SurR. Electromobility shift assay demonstrated that SurR reduced by the TrxR-Pdo system could bind to the DNA probe with the SurR-binding motif, GTTttgAAC. In this study, we present the TrxR-Pdo couple as a redox-regulator for SurR in T. onnurineus NA1. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER JAPAN KK | - |
dc.title | Redox regulation of SurR by protein disulfide oxidoreductase in Thermococcus onnurineus NA1 | - |
dc.type | Article | - |
dc.citation.endPage | 498 | - |
dc.citation.startPage | 491 | - |
dc.citation.title | EXTREMOPHILES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 3 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 정해창 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.identifier.bibliographicCitation | EXTREMOPHILES, v.21, no.3, pp.491 - 498 | - |
dc.identifier.doi | 10.1007/s00792-017-0919-1 | - |
dc.identifier.scopusid | 2-s2.0-85014091404 | - |
dc.identifier.wosid | 000400233700006 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | ARCHAEON PYROCOCCUS-FURIOSUS | - |
dc.subject.keywordPlus | GLUTAREDOXIN-LIKE PROTEIN | - |
dc.subject.keywordPlus | BOND FORMATION | - |
dc.subject.keywordPlus | SULFOLOBUS-SOLFATARICUS | - |
dc.subject.keywordPlus | THIOREDOXIN REDUCTASE | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | ELEMENTAL SULFUR | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordAuthor | Protein disulfide oxidoreductase | - |
dc.subject.keywordAuthor | Thioredoxin reductase | - |
dc.subject.keywordAuthor | SurR | - |
dc.subject.keywordAuthor | Thermococcus onnurineus NA1 | - |
dc.subject.keywordAuthor | Redox system | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Microbiology | - |