Identification and large isolation of an anti-inflammatory compound from an edible brown seaweed, Undariopsis peterseniana, and evaluation on its anti-inflammatory effect in in vitro and in vivo zebrafish SCIE SCOPUS

Cited 9 time in WEB OF SCIENCE Cited 8 time in Scopus
Title
Identification and large isolation of an anti-inflammatory compound from an edible brown seaweed, Undariopsis peterseniana, and evaluation on its anti-inflammatory effect in in vitro and in vivo zebrafish
Author(s)
Lee, Ji-Hyeok; Ko, Ju-Young; Kim, Eun-A; Hwang, Eun-Kyoung; Park, Chan Sun; Lee, Jung-Suck; Kim, Chul-Young; Lee, Hyi-Seung; Kang, Hee-Kyoung; Cha, Seon-Heui; Jeon, You-Jin
KIOST Author(s)
Lee, Hyi Seung(이희승)
Publication Year
2017-06
Abstract
In this study, we isolated and purified secondary metabolites from an edible brown seaweed, Undariopsis peterseniana, and evaluated their anti-inflammatory effects in vitro and in vivo. Apo-9'-fucoxanthinone (Apo-9'), a main anti-inflammatory compound found in the chloroform fraction of U. peterseniana extracts, was first isolated by one-step centrifugal partition chromatography and identified by nuclear magnetic resonance. Apo-9' significantly decreased nitric oxide (NO), prostaglandin E-2, pro-inflammatory proteins (inducible NO synthase [iNOS] and cyclooxygenase [COX-2]), and pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells without exhibiting any cytotoxicity. In vivo, Apo-9' protected LPS-stimulated zebrafish embryos against inflammatory stress and suppressed the expression of pro-inflammatory proteins such as COX-2 and iNOS. These findings suggest that U. peterseniana extract and Apo-9' could be used as an anti-inflammatory agent in health care.
ISSN
0921-8971
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1233
DOI
10.1007/s10811-016-1012-3
Bibliographic Citation
JOURNAL OF APPLIED PHYCOLOGY, v.29, no.3, pp.1587 - 1596, 2017
Publisher
SPRINGER
Subject
INDUCED INFLAMMATORY RESPONSE; CENTRIFUGAL PARTITION CHROMATOGRAPHY; NF-KAPPA-B; PREPARATIVE ISOLATION; ECKLONIA-CAVA; ALGA; EXTRACT; PHLOROTANNINS; PURIFICATION; FRACTION
Keywords
Phaeophyceae, centrifugal partition chromatography; Inflammation; Apo-9 ' -fucoxanthinone; RAW 264.7 cell line; Zebrafish
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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