A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses SCIE SCOPUS

Cited 73 time in WEB OF SCIENCE Cited 75 time in Scopus
Title
A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses
Author(s)
Fernando, I. P. Shanura; Sanjeewa, K. K. Asanka; Samarakoon, Kalpa W.; Lee, Won Woo; Kim, Hyun-Soo; Kang, Nalae; Ranasinghe, P.; Lee, Hyi-Seung; Jeon, You-Jin
KIOST Author(s)
Lee, Hyi Seung(이희승)
Publication Year
2017-11
Abstract
Fucoidans are an interesting group of bioactive sulfated polysaccharides abundant in brown seaweeds. The current study highlights the enrichment and extraction of fucoidan from Chnoospora minima by means of enzyme-assistant extraction using Celluclast and evaluation of its anti-inflammatory potential through in vitro and in vivo studies. The purified C. minima fucoidan (F2,4) inhibited the nitrous oxide (NO) production (IC50 = 27.82 +/- 0.88 mu g/ml) and expression of PGE(2) through the subsequent downregulation of iNOS and COX-2 expression in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. F2,4 downregulated TNF-alpha, IL1-beta, and IL-6 in RAW 264.7 macrophages in a dose-dependent manner and suppressed NO and ROS production in LPS stimulated zebrafish embryos while exerting a protective effect against the cell damage caused by LPS. Polysaccharide structural characterization was performed using FTIR, HPAE-PAD analysis of the monosaccharide content and NMR spectroscopy. Current findings confirm the potential anti-inflammatory activity of fucoidan purified from C. minima and elaborate its potential application as a functional ingredient in consumer products. (C) 2017 Elsevier B.V. All rights reserved.
ISSN
0141-8130
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1129
DOI
10.1016/j.ijbiomac.2017.07.031
Bibliographic Citation
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.104, pp.1185 - 1193, 2017
Publisher
ELSEVIER SCIENCE BV
Subject
RAW 264.7 MACROPHAGES; ECKLONIA-CAVA; MARINE-ALGAE; SULFATED POLYSACCHARIDES; ANTIOXIDANT ACTIVITY; ZEBRAFISH MODEL; MACROALGAE; EXTRACTION; SEAWEEDS; FUCANS
Keywords
Chnoospora minima; Anti-inflammatory activity; Brown algae; Fucoidan
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
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