A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses
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Title
- A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses
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Author(s)
- Fernando, I. P. Shanura; Sanjeewa, K. K. Asanka; Samarakoon, Kalpa W.; Lee, Won Woo; Kim, Hyun-Soo; Kang, Nalae; Ranasinghe, P.; Lee, Hyi-Seung; Jeon, You-Jin
- KIOST Author(s)
- Lee, Hyi Seung(이희승)
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Alternative Author(s)
- 이희승
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Publication Year
- 2017-11
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Abstract
- Fucoidans are an interesting group of bioactive sulfated polysaccharides abundant in brown seaweeds. The current study highlights the enrichment and extraction of fucoidan from Chnoospora minima by means of enzyme-assistant extraction using Celluclast and evaluation of its anti-inflammatory potential through in vitro and in vivo studies. The purified C. minima fucoidan (F2,4) inhibited the nitrous oxide (NO) production (IC50 = 27.82 +/- 0.88 mu g/ml) and expression of PGE(2) through the subsequent downregulation of iNOS and COX-2 expression in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. F2,4 downregulated TNF-alpha, IL1-beta, and IL-6 in RAW 264.7 macrophages in a dose-dependent manner and suppressed NO and ROS production in LPS stimulated zebrafish embryos while exerting a protective effect against the cell damage caused by LPS. Polysaccharide structural characterization was performed using FTIR, HPAE-PAD analysis of the monosaccharide content and NMR spectroscopy. Current findings confirm the potential anti-inflammatory activity of fucoidan purified from C. minima and elaborate its potential application as a functional ingredient in consumer products. (C) 2017 Elsevier B.V. All rights reserved.
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ISSN
- 0141-8130
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/1129
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DOI
- 10.1016/j.ijbiomac.2017.07.031
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Bibliographic Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.104, pp.1185 - 1193, 2017
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Publisher
- ELSEVIER SCIENCE BV
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Subject
- RAW 264.7 MACROPHAGES; ECKLONIA-CAVA; MARINE-ALGAE; SULFATED POLYSACCHARIDES; ANTIOXIDANT ACTIVITY; ZEBRAFISH MODEL; MACROALGAE; EXTRACTION; SEAWEEDS; FUCANS
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Keywords
- Chnoospora minima; Anti-inflammatory activity; Brown algae; Fucoidan
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Type
- Article
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Language
- English
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Document Type
- Article
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