A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Fernando, I. P. Shanura | - |
dc.contributor.author | Sanjeewa, K. K. Asanka | - |
dc.contributor.author | Samarakoon, Kalpa W. | - |
dc.contributor.author | Lee, Won Woo | - |
dc.contributor.author | Kim, Hyun-Soo | - |
dc.contributor.author | Kang, Nalae | - |
dc.contributor.author | Ranasinghe, P. | - |
dc.contributor.author | Lee, Hyi-Seung | - |
dc.contributor.author | Jeon, You-Jin | - |
dc.date.accessioned | 2020-04-16T09:55:13Z | - |
dc.date.available | 2020-04-16T09:55:13Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/1129 | - |
dc.description.abstract | Fucoidans are an interesting group of bioactive sulfated polysaccharides abundant in brown seaweeds. The current study highlights the enrichment and extraction of fucoidan from Chnoospora minima by means of enzyme-assistant extraction using Celluclast and evaluation of its anti-inflammatory potential through in vitro and in vivo studies. The purified C. minima fucoidan (F2,4) inhibited the nitrous oxide (NO) production (IC50 = 27.82 +/- 0.88 mu g/ml) and expression of PGE(2) through the subsequent downregulation of iNOS and COX-2 expression in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. F2,4 downregulated TNF-alpha, IL1-beta, and IL-6 in RAW 264.7 macrophages in a dose-dependent manner and suppressed NO and ROS production in LPS stimulated zebrafish embryos while exerting a protective effect against the cell damage caused by LPS. Polysaccharide structural characterization was performed using FTIR, HPAE-PAD analysis of the monosaccharide content and NMR spectroscopy. Current findings confirm the potential anti-inflammatory activity of fucoidan purified from C. minima and elaborate its potential application as a functional ingredient in consumer products. (C) 2017 Elsevier B.V. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | RAW 264.7 MACROPHAGES | - |
dc.subject | ECKLONIA-CAVA | - |
dc.subject | MARINE-ALGAE | - |
dc.subject | SULFATED POLYSACCHARIDES | - |
dc.subject | ANTIOXIDANT ACTIVITY | - |
dc.subject | ZEBRAFISH MODEL | - |
dc.subject | MACROALGAE | - |
dc.subject | EXTRACTION | - |
dc.subject | SEAWEEDS | - |
dc.subject | FUCANS | - |
dc.title | A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses | - |
dc.type | Article | - |
dc.citation.endPage | 1193 | - |
dc.citation.startPage | 1185 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 104 | - |
dc.contributor.alternativeName | 이희승 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.104, pp.1185 - 1193 | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2017.07.031 | - |
dc.identifier.scopusid | 2-s2.0-85022216229 | - |
dc.identifier.wosid | 000412959300133 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | RAW 264.7 MACROPHAGES | - |
dc.subject.keywordPlus | ECKLONIA-CAVA | - |
dc.subject.keywordPlus | MARINE-ALGAE | - |
dc.subject.keywordPlus | SULFATED POLYSACCHARIDES | - |
dc.subject.keywordPlus | ANTIOXIDANT ACTIVITY | - |
dc.subject.keywordPlus | ZEBRAFISH MODEL | - |
dc.subject.keywordPlus | MACROALGAE | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | SEAWEEDS | - |
dc.subject.keywordPlus | FUCANS | - |
dc.subject.keywordAuthor | Chnoospora minima | - |
dc.subject.keywordAuthor | Anti-inflammatory activity | - |
dc.subject.keywordAuthor | Brown algae | - |
dc.subject.keywordAuthor | Fucoidan | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |