Ciona intestinalis calcitonin-like peptide promotes osteoblast differentiation and mineralization through MAPK pathway in MC3T3-E1 cells SCIE SCOPUS

Cited 6 time in WEB OF SCIENCE Cited 6 time in Scopus
Title
Ciona intestinalis calcitonin-like peptide promotes osteoblast differentiation and mineralization through MAPK pathway in MC3T3-E1 cells
Author(s)
Van-Tinh Nguyen; Ko, Seok-Chun; Heo, Soo-Jin; Kang, Do-Hyung; Oh, Chulhong; Kim, Kil-Nam; Jeon, You-Jin; Kim, Young-Mog; Park, Won Sun; Choi, Il-Whan; Park, Nam Gyu; Jung, Won-Kyo
KIOST Author(s)
Heo, Soo Jin(허수진)Kang, Do Hyung(강도형)Oh, Chul Hong(오철홍)
Publication Year
2018-04
Abstract
This study examined the effects of Ciona intestinalis calcitonin-like peptide (CCLP) on osteoblast differentiation and mineralization in the culture system of MC3T3-E1 cells. The primary structure of the CCLP containing Cys-Asp-Gly-Val-Ser-Thr-Cys-Trp-Leu-His-Glu-Leu-Gly-Asn-Ser-Val-His-Ala-Thr-Ala-Gly-Gly-Lys-Gln-Asn-Val-Gly- Phe-Gly-Pro-NH2 was synthesized automatically using the solid phase method with fluorenylmethoxycarbonyl (Fmoc) resin. Pre-osteoblast MC3T3-E1 cells were cultured with various concentrations of CCLP (7.5, 15, and 30 mu M) during the osteoblast differentiation period. To examine osteoblast differentiation, alkaline phosphatase (ALP) activity was determined by reading the absorbance at 405 nm using a spectrophotometer, and mineralization was evaluated by staining with Alizalin red S. Moreover, the expression of differentiation markers such as ALP, osteocalcin, and osteopontin was measured using RT-PCR and Western blot analysis. The results showed that CCLP did not exhibit any cytotoxic effect on MC3T3-E1 cells even at the highest concentration (30 pM) at 2 and 5 days. CCLP also enhanced MC3T3-E1 cells proliferation, differentiation, and mineralization demonstrated by the increased expression of several osteoblast phenotype markers such as ALP, and Alizarin Red S staining. In addition, the CCLP induced mitogen-activated protein kinase (MAPK) pathway in MC3T3-E1 cells. These results suggest that CCLP exerts positive effects on osteoblast differentiation and may represent a potential target for pharmaceutical development.
ISSN
1359-5113
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/985
DOI
10.1016/j.procbio.2018.01.025
Bibliographic Citation
PROCESS BIOCHEMISTRY, v.67, pp.127 - 138, 2018
Publisher
ELSEVIER SCI LTD
Subject
MESENCHYMAL STEM-CELLS; GENE-RELATED PEPTIDE; MORPHOGENETIC PROTEIN-2 EXPRESSION; PERIODONTAL-LIGAMENT CELLS; OSTEOGENIC DIFFERENTIATION; BONE-MARROW; IN-VITRO; ACTIVATION; GROWTH; VIVO
Keywords
Ciona intestinalis; Calcitonin-like peptide; MAPK pathway; MC3T3-E1 cells; Osteoblast differentiation
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
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