Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells SCIE SCOPUS

Cited 8 time in WEB OF SCIENCE Cited 9 time in Scopus
Title
Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells
Author(s)
Choudhary, Indu; Lee, Hyunkyoung; Pyo, Min Jung; Heo, Yunwi; Chae, Jinho; Yum, Seung Shic; Kang, Changkeun; Kim, Euikyung
KIOST Author(s)
Yum, Seung Shic(염승식)
Alternative Author(s)
염승식
Publication Year
2018-05
Abstract
Nemopilema nomurai is a giant jellyfish that blooms in East Asian seas. Recently, N. nomurai venom (NnV) was characterized from a toxicological and pharmacological point of view. A mild dose of NnV inhibits the growth of various kinds of cancer cells, mainly hepatic cancer cells. The present study aims to identify the potential therapeutic targets and mechanism of NnV in the growth inhibition of cancer cells. Human hepatocellular carcinoma (HepG2) cells were treated with NnV, and its proteome was analyzed using two-dimensional gel electrophoresis, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF/MS). The quantity of twenty four proteins in NnV-treated HepG2 cells varied compared to non-treated control cells. Among them, the amounts of fourteen proteins decreased and ten proteins showed elevated levels. We also found that the amounts of several cancer biomarkers and oncoproteins, which usually increase in various types of cancer cells, decreased after NnV treatment. The representative proteins included proliferating cell nuclear antigen (PCNA), glucose-regulated protein 78 (GRP78), glucose-6-phosphate dehydrogenase (G6PD), elongation factor 1 (EF1), nucleolar and spindle-associated protein (NuSAP), and activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1). Western blotting also confirmed altered levels of PCNA, GRP78, and G6PD in NnV-treated HepG2 cells. In summary, the proteomic approach explains the mode of action of NnV as an anticancer agent. Further characterization of NnV may help to unveil novel therapeutic agents in cancer treatment.
ISSN
2072-6651
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/915
DOI
10.3390/toxins10050194
Bibliographic Citation
TOXINS, v.10, no.5, 2018
Publisher
MDPI
Subject
MESSENGER-RNA EXPRESSION; KAPPA-B PATHWAY; CANCER-CELLS; HEPATOCELLULAR-CARCINOMA; GENE-EXPRESSION; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE EXPRESSION; ESOPHAGEAL-CARCINOMA; PANCREATIC-CANCER; GASTRIC-CARCINOMA; POOR-PROGNOSIS
Keywords
jellyfish; Nemopilema nomurai; HepG2 cell; venom; proteomics; MALDI/TOF/MS; 2-DE
Type
Article
Language
English
Document Type
Article
Publisher
MDPI
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