3-Chloro-4,5-dihydroxybenzaldehyde inhibits adipogenesis in 3T3-L1 adipocytes by regulating expression of adipogenic transcription factors and AMPK activation

Kang, Min-Cheol; Ding, Yuling; Kim, Junseong; Kim, Eun-A; Fernando, I. P. Shanura; Heo, Soo-Jin; Lee, Seung-Hong

doi:10.1016/j.cbi.2018.04.001

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3-Chloro-4,5-dihydroxybenzaldehyde inhibits adipogenesis in 3T3-L1 adipocytes by regulating expression of adipogenic transcription factors and AMPK activation SCIE SCOPUS

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Title: 3-Chloro-4,5-dihydroxybenzaldehyde inhibits adipogenesis in 3T3-L1 adipocytes by regulating expression of adipogenic transcription factors and AMPK activation

Author(s): Kang, Min-Cheol; Ding, Yuling; Kim, Junseong; Kim, Eun-A; Fernando, I. P. Shanura; Heo, Soo-Jin; Lee, Seung-Hong

KIOST Author(s): Kim, Jun Seong(김준성); Kim, Eun A(김은아); Heo, Soo Jin(허수진)

Alternative Author(s): 김준성; 김은아; 허수진

Publication Year: 2018-05-01

Abstract: Obesity is a serious health issue in many industrialized countries. It is a medical condition with excessive levels of fat accumulated in adipocytes. The objective of the present study was to determine the inhibitory effect of 3-chloro-4,5-dihydroxybenzaldehyde (CDB) on adipogenesis in 3T3-L1 adipocyte cells. CDB suppressed the differentiation and decreased lipid accumulation and triglycerides contents in 3T3-L1 adipocytes. Its suppression effect on fat accumulation was mediated via expression of adipogenesis factors (C/EBP alpha, SREBP-1c, PPAR gamma, and adiponectin) during adipocyte differentiation in white adipocyte cells. CDB's ability to suppress fat accumulation was increased in a concentration-dependent manner. It inhibited fatty acid synthesis related proteins including FAS, FABP4, leptin, and perilipin. It also increased expression of phosphorylated AMPK in adipocytes cells. These observations suggest that CDB has potential anti-obesity effect with ability to improve metabolic diseases.