Dynamics of Amoebophrya parasites during recurrent blooms of the ichthyotoxic dinoflagellate Cochlodinium polykrikoides in Korean coastal waters SCIE SCOPUS

Cited 11 time in WEB OF SCIENCE Cited 12 time in Scopus
Title
Dynamics of Amoebophrya parasites during recurrent blooms of the ichthyotoxic dinoflagellate Cochlodinium polykrikoides in Korean coastal waters
Author(s)
Park, Bum Soo; Kim, Sunju; Kim, Joo-Hwan; Kim, Jin Ho; Han, Myung-Soo
Publication Year
2019-04
Abstract
During the bloom events of the harmful dinoflagellate Cochlodinium polykrikoides in August and October, 2012, infections by two different Amoebophrya species were observed in Korean coastal waters. To investigate the dynamics of the two parasites and their relative impact on the host populations, a quantitative real-time PCR (qPCR) method was applied to detect and quantify the parasites in the free-living and parasitic stages. Each specific primer set of the target species, Amoebophrya sp. 1 and sp. 2 was designed on the large subunit (LSU) and the first internal transcribed spacer (ITS1) of ribosomal RNA (rRNA) gene, respectively. Dynamics of the two Amoebophrya species via qPCR assay showed distinct patterns during the C. polykrikoides bloom events. Amoebophrya sp. 1 showed peaks during both bloom events in August and October with relatively low copies (10(6) to 10(7) copies L-1), while Amoebophrya sp. 2 appeared only during the bloom event in October with very high copies (10(9) to 10(10) copies L-1). Overall, the qPCR measurements for the dynamics of two Amoebophrya species in the parasitic stage ( > 5 mu m fractions) were consistent with parasite prevalence through microscopic observations. Amoebophrya sp. 1 infections were observed during both bloom events in August and October with relatively low parasite prevalence (0.1-1.5%), while Amoebophrya sp. 2 infections were detected only during the bloom event in October with high prevalence (up to 45%). Taken together, Amoebophrya sp. 1 may be a generalist and C. polykrikoides may not be its primary host, while Amoebophrya sp. 2 may be a specialist which can substantially impact host population dynamics.
ISSN
1568-9883
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/663
DOI
10.1016/j.hal.2019.02.001
Bibliographic Citation
HARMFUL ALGAE, v.84, pp.119 - 126, 2019
Publisher
ELSEVIER SCIENCE BV
Subject
REAL-TIME PCR; PERKINSUS-MARINUS; QUANTIFICATION; SUBPOPULATIONS; DINOPHYCEAE; SUCCESSION; MICROALGAE; GENERATION; INHIBITION; STRATEGIES
Keywords
Amoebophrya; Bloom dynamics; Cochlodiniumpolykrikoides; Parasitism; Uantitative real-time PCR
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse