Polymorphisms of the lipoprotein lipase gene of red seabream, Pagrus major
SCOPUS
KCI
-
Title
- Polymorphisms of the lipoprotein lipase gene of red seabream, Pagrus major
-
Author(s)
- Jang, Y.-S.; Hong, K.P.; Noh, C.H.
- KIOST Author(s)
- Jang, Yo Soon(장요순); Noh, Choong Hwan(노충환)
-
Alternative Author(s)
- 장요순; 홍경표; 노충환
-
Publication Year
- 2004-12
-
Abstract
- Polymorphism of the lipoprotein lipase (LPL) gene which plays an important role in regulation of lipid deposition was analysed in two red seabream (pagrus major) populations (KF4, cultured KORDI line, n = 100; JPN, imported from Japan, n = 100). We amplified a DNA fragment (1,091 bp) including the exon 2 region of the LPL gene, and conducted PCR-RFLP analysis using MspI and AluI. The PCR products were also sequenced. Two alleles (A and B) were found in MspI digestion and five alleles (A, B, C, D and E) in AluI digestion. The sequenced data revealed four nucleotide substitutions including one transversion at the MspI recognition site (nt 2,235, C→G) and three transitions at the AluI recognition sites (nt 1,727, A→G; nt 2,319, C→T; nt 2,319, T→C. Among them, substitutions at the nt 2,235 and 2,319 sites which are located in the exon 2 were proved to be silent point mutations. MspI polymorphism resulted in 3 genotypes, and the allele frequency was significantly different between the two fish populations, KF4 and JPN. In the case of AluI polymorphism, the 5 alleles (A, B, C, D, E) comprised 12 genotypes of the 5 alleles. KF4 population, alleles D and E were specific to the LPL gene polymorphisms would be useful DNA markers for red seabream population.
-
ISSN
- 1598-141X
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/6518
-
DOI
- 10.4217/OPR.2004.26.4.551
-
Bibliographic Citation
- Ocean and Polar Research, v.26, no.4, pp.551 - 557, 2004
-
Publisher
- Korea Ocean Research and Development Institute
-
Keywords
- DNA marker; Lipoprotein lipase, LPL; Pagrus major; PCR-RFLP; Silent point mutation
-
Type
- Article
-
Language
- Korean
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.