Inhibition of DNA topoisomerase I by dihydrotanshinone I, components of a medicinal herb Salvia miltiorrhiza bunge
SCIE
SCOPUS
Cited 12 time in
WEB OF SCIENCE
Cited 14 time in
Scopus
-
Title
- Inhibition of DNA topoisomerase I by dihydrotanshinone I, components of a medicinal herb Salvia miltiorrhiza bunge
-
Author(s)
- Lee, DS; Lee, SH; Kwon, GS; Lee, HK; Woo, JH; Kim, JG; Hong, SD
-
Alternative Author(s)
- 이홍금
-
Publication Year
- 1999-08
-
Abstract
- Dihydrotanshinone I induced topoisomerase I-mediated DNA cleavage in vitro as strongly as camptothecin, but topoisomerase II-mediated DNA cleavage was not affected. In a DNA relaxation assay using calf thymus DNA topoisomerase I and supercoiled pBR322 plasmid DNA, dihydrotanshinone I reduced topoisomerase I-mediated DNA relaxation in a dose-dependent manner. Heat treatment (65 degrees C) of the reaction mixture containing dihydrotanshinone I and topoisomerase I resulted in a substantial reduction in DNA cleavage, suggesting topoisomerase I and dihydrotanshinone I may form a reversible cleavable complex to induce DNA damage. A DNA unwinding assay using T-4 DNA ligase showed that dihydrotanshinone I is a very weak DNA intercalator. These results suggest that dihydrotanshinone I inhibits the catalytic activity of topoisomerase I by the formation of a cleavable complex and at least in part through the intercalation into DNA.
-
ISSN
- 0916-8451
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/6157
-
DOI
- 10.1271/bbb.63.1370
-
Bibliographic Citation
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.63, no.8, pp.1370 - 1373, 1999
-
Publisher
- TAYLOR & FRANCIS LTD
-
Subject
- ANTITUMOR DRUGS; CAMPTOTHECIN; CLEAVAGE; INDUCTION; BREAKS
-
Keywords
- dihydrotanshinone I; DNA topoisomerase I inhibitor; DNA intercalator
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.