Inhibition of DNA topoisomerase I by dihydrotanshinone I, components of a medicinal herb Salvia miltiorrhiza bunge SCIE SCOPUS

Cited 11 time in WEB OF SCIENCE Cited 14 time in Scopus
Title
Inhibition of DNA topoisomerase I by dihydrotanshinone I, components of a medicinal herb Salvia miltiorrhiza bunge
Author(s)
Lee, DS; Lee, SH; Kwon, GS; Lee, HK; Woo, JH; Kim, JG; Hong, SD
Publication Year
1999-08
Abstract
Dihydrotanshinone I induced topoisomerase I-mediated DNA cleavage in vitro as strongly as camptothecin, but topoisomerase II-mediated DNA cleavage was not affected. In a DNA relaxation assay using calf thymus DNA topoisomerase I and supercoiled pBR322 plasmid DNA, dihydrotanshinone I reduced topoisomerase I-mediated DNA relaxation in a dose-dependent manner. Heat treatment (65 degrees C) of the reaction mixture containing dihydrotanshinone I and topoisomerase I resulted in a substantial reduction in DNA cleavage, suggesting topoisomerase I and dihydrotanshinone I may form a reversible cleavable complex to induce DNA damage. A DNA unwinding assay using T-4 DNA ligase showed that dihydrotanshinone I is a very weak DNA intercalator. These results suggest that dihydrotanshinone I inhibits the catalytic activity of topoisomerase I by the formation of a cleavable complex and at least in part through the intercalation into DNA.
ISSN
0916-8451
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/6157
DOI
10.1271/bbb.63.1370
Bibliographic Citation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.63, no.8, pp.1370 - 1373, 1999
Publisher
TAYLOR & FRANCIS LTD
Subject
ANTITUMOR DRUGS; CAMPTOTHECIN; CLEAVAGE; INDUCTION; BREAKS
Keywords
dihydrotanshinone I; DNA topoisomerase I inhibitor; DNA intercalator
Type
Article
Language
English
Document Type
Article
Publisher
TAYLOR & FRANCIS LTD
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse