Intracellular protons are not involved in elicitor dependent regulation of mRNAs for defence related enzymes in Chenopodium rubrum SCIE SCOPUS

Cited 4 time in WEB OF SCIENCE Cited 0 time in Scopus
Title
Intracellular protons are not involved in elicitor dependent regulation of mRNAs for defence related enzymes in Chenopodium rubrum
Author(s)
Hofmann, M; Ehness, R; Lee, TK; Roitsch, T
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
1999-10
Abstract
Protons have been implicated in the signal transduction of elicitor-generated defence reactions. To address the possible function of cytosolic acidification we determined both intracellular and extracellular pH values and phenylalanine-ammonia-lyase (PAL) and extracellular invertase (CIN1) mRNA levels of photoautotrophic suspension culture Chenopodium rubrum cells in response to a number of stimuli known to elicit defence responses. The results of these experiments show that there is no principle link between elicitor-induced persistent pH-shifts and the induction of the defence related mRNAs. Treatment with the fungal elicitor chitosan led to intracellular acidification, extracellular alkalinisation, and a rise of both PAL and CIN1 mRNA levels. However, when these pH shifts are reversed by treatment with fusicoccin, a known activator of the plasma membrane ATPase, both PAL and CIN 1 mRNA levels are also elevated. A comparison between the kinetics of intracellular acidification and the rise in PAL and CIN 1 mRNA levels in response to chitosan shows that the activation of these genes precedes intracellular acidification by 1 h, indicating that protons are not involved in the analysed signal transduction pathway leading to the induction of PAL and CIN 1 mRNAs.
ISSN
0176-1617
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/6143
DOI
10.1016/S0176-1617(99)80049-2
Bibliographic Citation
JOURNAL OF PLANT PHYSIOLOGY, v.155, no.4-5, pp.527 - 532, 1999
Publisher
GUSTAV FISCHER VERLAG
Subject
EXTRACELLULAR ALKALINIZATION; TOBACCO CELLS; CYTOPLASMIC ACIDIFICATION; PROTEIN-PHOSPHORYLATION; SUSPENSION-CULTURES; SOYBEAN CELLS; TOMATO CELLS; PLANT-CELLS; PH; ELICITATION
Keywords
Chenopodium rubrum; elicitor; intracellular pH; extracellular pH; signal transduction
Type
Article
Language
English
Document Type
Article
Publisher
GUSTAV FISCHER VERLAG
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse