Intracellular protons are not involved in elicitor dependent regulation of mRNAs for defence related enzymes in Chenopodium rubrum
SCIE
SCOPUS
Cited 4 time in
WEB OF SCIENCE
Cited 0 time in
Scopus
-
Title
- Intracellular protons are not involved in elicitor dependent regulation of mRNAs for defence related enzymes in Chenopodium rubrum
-
Author(s)
- Hofmann, M; Ehness, R; Lee, TK; Roitsch, T
- KIOST Author(s)
- Lee, Taek Kyun(이택견)
-
Alternative Author(s)
- 이택견
-
Publication Year
- 1999-10
-
Abstract
- Protons have been implicated in the signal transduction of elicitor-generated defence reactions. To address the possible function of cytosolic acidification we determined both intracellular and extracellular pH values and phenylalanine-ammonia-lyase (PAL) and extracellular invertase (CIN1) mRNA levels of photoautotrophic suspension culture Chenopodium rubrum cells in response to a number of stimuli known to elicit defence responses. The results of these experiments show that there is no principle link between elicitor-induced persistent pH-shifts and the induction of the defence related mRNAs. Treatment with the fungal elicitor chitosan led to intracellular acidification, extracellular alkalinisation, and a rise of both PAL and CIN1 mRNA levels. However, when these pH shifts are reversed by treatment with fusicoccin, a known activator of the plasma membrane ATPase, both PAL and CIN 1 mRNA levels are also elevated. A comparison between the kinetics of intracellular acidification and the rise in PAL and CIN 1 mRNA levels in response to chitosan shows that the activation of these genes precedes intracellular acidification by 1 h, indicating that protons are not involved in the analysed signal transduction pathway leading to the induction of PAL and CIN 1 mRNAs.
-
ISSN
- 0176-1617
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/6143
-
DOI
- 10.1016/S0176-1617(99)80049-2
-
Bibliographic Citation
- JOURNAL OF PLANT PHYSIOLOGY, v.155, no.4-5, pp.527 - 532, 1999
-
Publisher
- GUSTAV FISCHER VERLAG
-
Keywords
- Chenopodium rubrum; elicitor; intracellular pH; extracellular pH; signal transduction
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.