Optimal protocol for enumeration of attached bacteria on glass slides

Abstract

In examining bacterial growth on glass surfaces immersed in sea water we found serious differences between enumeration methods. Therefore, we compared various methods and found sonication and direct count methods mere superior to other methods. Since the direct count method was not suitable for long-term investigation, we chose the sonication method and confirmed that sonication periods 8 times for 30 seconds was optimal for the detachment of bacteria from glass surfaces.