Quantification of reproductive output of the butter clam, Saxidomus purpuratus (Sowerby, 1852) using Enzyme-Linked Immunosorbent Assay (ELISA) SCOPUS KCI

Title
Quantification of reproductive output of the butter clam, Saxidomus purpuratus (Sowerby, 1852) using Enzyme-Linked Immunosorbent Assay (ELISA)
Author(s)
Park, K.-I.; Choi, J.-W.; Choi, K.-S.
Publication Year
2003
Abstract
An immunological method was developed in this study to quantify reproductive output of the female butter clam, Saxidomus purpuratus. A clam egg-specific polyclonal antibody was developed using the purified butter clam egg as an antigen. An indirect Enzyme-Linked Immunosorbent Assay (ELISA) was used in quantitative measurement of the eggs. Size of the butter clam eggs ranged from 70.81 ± 7.52 μm in histology or 88.56 ± 11.31 μm in intact eggs. The predominant egg constituent was protein (37.44%), followed by lipids (11.40%) and carbohydrates (9.68%). The SDS-PAGE showed that the egg proteins are composed of several peptides of molecular weights consisting of 247, 200, 99, 91, 54, and 47 kDa. ELISA indicated that the clams collected from Geoje Island in May 2002 produced 8.2 to 26.8% of their body weight as eggs or 9,307,309 to 31,156,333 with a mean of 16,931,893 eggs per individual clam. The results of this study thus suggest that indirect ELISA using rabbit anti-clam egg IgG as a primary antibody is a rapid, affordable and sensitive method to assess reproductive output of S. purpuratus and possibly other bivalves using a small amount of eggs.
ISSN
1598-141X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5576
DOI
10.4217/OPR.2003.25.3.249
Bibliographic Citation
Ocean and Polar Research, v.25, no.3, pp.249 - 256, 2003
Publisher
Korea Ocean Research and Development Institute
Subject
clam fishery; fecundity; immunoassay; reproductive productivity; Geoje Island; South Korea; Bivalvia; Bivalvia; Oryctolagus cuniculus; Saxidomus; Saxidomus giganteus; Saxidomus purpuratus; Veneridae; Veneridae
Keywords
Egg; ELISA; Fecundity; Immuno-assay; Korea; Reproduction; Reproductive output; Saxidomus purpuratus
Type
Article
Language
Korean
Document Type
Article
Publisher
Korea Ocean Research and Development Institute
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