A simple method for DNA extraction from marine bacteria that produce extracellular materials SCIE SCOPUS

Cited 93 time in WEB OF SCIENCE Cited 98 time in Scopus
Title
A simple method for DNA extraction from marine bacteria that produce extracellular materials
Author(s)
Lee, YK; Kim, HW; Liu, CL; Lee, HK
Publication Year
2003-02
Abstract
We present a simple method for extracting DNA from the marine bacteria Hahella chejuensis, a Streptomyces sp., and a Cytophaga sp. Previously, DNA purification from these strains was hindered by the presence of extracellular materials. In our extraction method, the marine bacteria are lysed by freezing and grinding in liquid nitrogen, and treated with SDS. The extracted DNA is purified using a phenol/chloroform mixture, and precipitated in isopropanol. The extracted DNA is of high quality and suitable for molecular analyses, such as PCR, restriction enzyme digestion, genomic DNA blot hybridization, and genomic DNA library construction. We used this method to extract genomic DNA from several other marine bacteria. Our method is a reproducible, simple, and rapid technique for routine DNA extractions from marine bacteria. Furthermore, the low cost of this method makes it attractive for large-scale studies. (C) 2003 Elsevier Science B.V. All rights reserved.
ISSN
0167-7012
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5555
DOI
10.1016/S0167-7012(02)00180-X
Bibliographic Citation
JOURNAL OF MICROBIOLOGICAL METHODS, v.52, no.2, pp.245 - 250, 2003
Publisher
ELSEVIER SCIENCE BV
Subject
AEROMONAS-SALMONICIDA; PURIFICATION; SOIL; LIPOPOLYSACCHARIDE; BIOSYNTHESIS; EXPRESSION; PROTEINS; CLONING; ALGAL
Keywords
Cytophaga; DNA extraction; Hahella; marine bacteria; Streptomyces
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
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