A Simple Method for DNA Extraction from Red Algae KCI OTHER

Title
A Simple Method for DNA Extraction from Red Algae
Alternative Title
A Simple Method for DNA Extraction from Red Algae
Author(s)
이유경; 이홍금
Publication Year
2003-03
Abstract
A simple method is described for extracting DNA from marine red algae from which it has been difficult to isolate DNA because of their polysaccharides and phenolic compounds. In this DNA extraction method, the red algae were lysed mechanically by freezing and grinding in liquid nitrogen, and chemically by SDS. Then, the DNA was purified in phenol/chloroform, and precipitated in isopropanol. This method was applied to Antithamnion sparsum, Campylaephora crassa, Gelidium amansii, Griffithsia japonica, Polysiphonia morrowii, Porphyra yezoensis, and Symphyocladia latiuscula. The DNA extracted by this method is high yield and high quality for molecular analyses, such as PCR, sequencing, restriction enzyme digestion, and genomic DNA library construction. This method is reproducible, simple, and fast for routine DNA extraction from red algae.
ISSN
1226-2617
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5539
Bibliographic Citation
ALGAE, v.18, no.1, pp.65 - 69, 2003
Publisher
한국조류학회I
Keywords
Antithamnion; Campylaephora; DNA extraction; Gelidium; Griffithsia; Polysiphonia; Porphyra; Symphyocladia
Type
Article
Language
English
Publisher
한국조류학회I
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