Pyrene과 Benzo(a)pyrene에 노출된 굴의 혈구세포와 아가미 세포에서의 DNA 손상 측정을 위한 Comet assay의 이용 KCI OTHER

Title
Pyrene과 Benzo(a)pyrene에 노출된 굴의 혈구세포와 아가미 세포에서의 DNA 손상 측정을 위한 Comet assay의 이용
Alternative Title
Use of the Comet Assay to Assess DNA Damage in Hemocytes and Gillof Oyster(Crassostrea gigas) Exposed to Pyrene and Benzo(a)pyrene
Author(s)
김기범; 배세진
Publication Year
2003-08
Abstract
Sessile organisms such as the oyster Crassostrea gigas have been given much attention as a potential biomonitoring indicator to assess the impact of toxicants on aquatic organism. In this study, we exposed cells isolated from gill of oyster (Crassostrea gigas) to hydrogen peroxide in vitro. In addition oysters were in vivo exposed to pyrene and benzo(a)pyrene at various concentrations for 2 weeks. Comet assay was used to detect DNA single strand breaks and to investigate the application of this technique as a tool for aquatic biomonitoring. Hydrogen peroxide increased DNA single strand break with increasing concentration after 30 minutes exposure in vitro. Pyrene and benzo(a)pyrene caused DNA damage only at very high concentration (100 μg /L or 1000 μg /L) at two week exposure in vivo. DNA damage was relatively higher at hemocyte than at gill. It suggested that metabolized PAHs are transferred to hemolymph from digestive gland which have a relatively high enzyme activity, and attacked the DNA of hemocyte, while gill accumulated PAHs without degrading them to their metabolites due to low enzyme activity at gill. Both in vitro and in vivo exposure experiments showed that the comet assay is an effective tool on screening whether the organism are exposed to genotoxic contaminants.
ISSN
1226-0193
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5467
Bibliographic Citation
한국양식학회지, v.16, no.3, pp.196 - 201, 2003
Publisher
한국양식학회
Keywords
DNA damage; Comet assay; Oyster; Crassostrea gigas; Hemocyte; Gill; Benzo(a)pyrene; Pyrene
Type
Article
Publisher
한국양식학회
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