Freezing seawater for the long-term storage of bacterial cells for mcroscopic enumeration SCIE SCOPUS KCI

DC Field Value Language
dc.contributor.author Hyun, JH -
dc.contributor.author Yang, EJ -
dc.date.accessioned 2020-04-21T06:25:07Z -
dc.date.available 2020-04-21T06:25:07Z -
dc.date.created 2020-01-28 -
dc.date.issued 2003-09 -
dc.identifier.issn 1225-8873 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/5459 -
dc.description.abstract Although enumerating bacterial cells is a fundamental step in understanding microbial ecosystems in marine environments, substantial decrease in bacterial counts with increasing sample storage time hampers the accurate estimation of bacterial biomass. We compared the variations in bacterial cell numbers caused by freezing and thawing of sample bottles or slides. Bacterial counts of seawater samples frozen only once in a sampling bottle yielded approximately 95% of the original numbers after 90 days, whereas 80% of the original count was obtained for samples prepared on slides. Only 67% and 58% of the original counts were recovered in samples repeatedly frozen and thawed in bottles or. on slides, respectively. The results indicated that freezing a seawater sample in a bottle increased the consistency of the epifluorescence microscopic enumeration of bacterial cells. -
dc.description.uri 1 -
dc.language English -
dc.publisher MICROBIOLOGY SOC KOREA -
dc.subject SARGASSO SEA -
dc.subject FILTERS -
dc.subject SAMPLES -
dc.title Freezing seawater for the long-term storage of bacterial cells for mcroscopic enumeration -
dc.type Article -
dc.citation.endPage 265 -
dc.citation.startPage 262 -
dc.citation.title JOURNAL OF MICROBIOLOGY -
dc.citation.volume 41 -
dc.citation.number 3 -
dc.contributor.alternativeName 현정호 -
dc.identifier.bibliographicCitation JOURNAL OF MICROBIOLOGY, v.41, no.3, pp.262 - 265 -
dc.identifier.wosid 000185657000015 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus SARGASSO SEA -
dc.subject.keywordPlus FILTERS -
dc.subject.keywordPlus SAMPLES -
dc.subject.keywordAuthor freezing seawater -
dc.subject.keywordAuthor storage of bacterial cells -
dc.subject.keywordAuthor microscopic enumeration -
dc.relation.journalWebOfScienceCategory Microbiology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.description.journalRegisteredClass kci -
dc.relation.journalResearchArea Microbiology -
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