Freezing seawater for the long-term storage of bacterial cells for mcroscopic enumeration SCIE SCOPUS KCI

Cited 12 time in WEB OF SCIENCE Cited 0 time in Scopus
Title
Freezing seawater for the long-term storage of bacterial cells for mcroscopic enumeration
Author(s)
Hyun, JH; Yang, EJ
Publication Year
2003-09
Abstract
Although enumerating bacterial cells is a fundamental step in understanding microbial ecosystems in marine environments, substantial decrease in bacterial counts with increasing sample storage time hampers the accurate estimation of bacterial biomass. We compared the variations in bacterial cell numbers caused by freezing and thawing of sample bottles or slides. Bacterial counts of seawater samples frozen only once in a sampling bottle yielded approximately 95% of the original numbers after 90 days, whereas 80% of the original count was obtained for samples prepared on slides. Only 67% and 58% of the original counts were recovered in samples repeatedly frozen and thawed in bottles or. on slides, respectively. The results indicated that freezing a seawater sample in a bottle increased the consistency of the epifluorescence microscopic enumeration of bacterial cells.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5459
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.41, no.3, pp.262 - 265, 2003
Publisher
MICROBIOLOGY SOC KOREA
Subject
SARGASSO SEA; FILTERS; SAMPLES
Keywords
freezing seawater; storage of bacterial cells; microscopic enumeration
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGY SOC KOREA
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