Molecular cloning of the translation elongation factor 1A (eEF1A) gene from the soft coral, Scleronephthya gracillimum (Kukenthal) and implication on Cnidarian Phylogeny SCIE SCOPUS

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Title
Molecular cloning of the translation elongation factor 1A (eEF1A) gene from the soft coral, Scleronephthya gracillimum (Kukenthal) and implication on Cnidarian Phylogeny
Author(s)
Woo, S; Yoon, M; Suh, DS; Song, JI; Kim, JH; Yum, S
KIOST Author(s)
Yum, Seung Shic(염승식)
Publication Year
2004-12
Abstract
The complete cDNA of eukaryotic translation elongation factor 1A (eEF1A) from the soft coral Scleronephthya gracillimum (Kukenthal) (Alcyonacea, Octocorallia, Anthozoa, Cnidaria) was cloned by RT-PCR and the nucleotide sequences were determined. The information on primary structure of the eEF1A gene and protein may be useful for the gene expression study as well as for the phylogenetic study. The full-length cDNA of S. gracillimum eEF1A comprised 1612 bp, not including the poly (A)(+) stretch, and contained a complete open reading frame encoding 461 amino acids. The deduced amino acid sequence showed 85% identity to placozoan, 86% to human and 77% to hydra. Considering that functional domains are highly conserved throughout the evolutionary history, the present study suggests that the amino acid sequence of eEF1A alone may not resolve the phylogenetic relationship of lower metazoans. A possible solution to overcome the long branch problems that appear in cnidiarian phylogeny is discussed.
ISSN
0254-5934
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5192
Bibliographic Citation
KOREAN JOURNAL OF GENETICS, v.26, no.4, pp.387 - 395, 2004
Publisher
GENETICS SOC KOREA
Subject
AMINO-ACID-SEQUENCE; NUCLEOTIDE-SEQUENCE; FACTOR EF-1-ALPHA; ANIMAL PHYLOGENY; EARLY EVOLUTION; PROTEIN; FACTOR-1-ALPHA; EUKARYOTES; ACTIN; INSECTA
Keywords
eEF1A; cDNA; Scleronephthya gracillimum; anthozoa; Cnidaria; phylogeny
Type
Article
Language
English
Document Type
Article
Publisher
GENETICS SOC KOREA
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

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