Hydrolysis of triglycerides with cold-adapted lipase of Psychrobacter sp. S3 isolated from intertidal flat SCOPUS KCI

DC Field Value Language
dc.contributor.author Lee, S.-A. -
dc.contributor.author Lee, J.-H. -
dc.contributor.author Kim, S.-J. -
dc.contributor.author Kim, H.-K. -
dc.date.accessioned 2020-04-20T14:40:13Z -
dc.date.available 2020-04-20T14:40:13Z -
dc.date.created 2020-01-28 -
dc.date.issued 2005 -
dc.identifier.issn 1598-642X -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/5133 -
dc.description.abstract Lipase-producing bacteria (S3) were isolated from intertidal flat at Saemanguem. A isolated strain was identified as Psychrobacter species by physiological and fermentational characterization as well as 16S rRNA analysis. The strain was then named as Psychrobacter sp. S3. P. sp. S3 grew most rapidly at 30°C, but grew well even at 10°C and its lipase activity was most high when cultivated at 20°C. Lipase S3 had optimum temperature of 30°C for the hydrolysis of p-nitrophenyl caproate and had more than 80% activity even at 10°C. The activation energy was calculated to be 1.5 kcal/mol, which showed that it was a typical cold-adapted enzyme. It was an alkaline enzyme with optimum pH of 9.0≃9.5. It could hydrolyze various length of triglycerides. Among them, it hydrolyzed most rapidly C4, C14, C 16-length triglycerides. When added to tributyrin-agarose gel, lipase S3 hydrolyzed tributyrin most rapidly at 30 and 40°C, but it could hydrolyze well even at 4°C. -
dc.description.uri 1 -
dc.language Korean -
dc.subject 4 nitrophenyl hexanoate -
dc.subject agarose -
dc.subject hexanoic acid derivative -
dc.subject RNA 16S -
dc.subject triacylglycerol -
dc.subject triacylglycerol lipase -
dc.subject tributyrin -
dc.subject unclassified drug -
dc.subject alkalinity -
dc.subject article -
dc.subject bacterial growth -
dc.subject bacterial strain -
dc.subject bacterium culture -
dc.subject bacterium identification -
dc.subject bacterium isolate -
dc.subject bacterium isolation -
dc.subject calculation -
dc.subject cold acclimatization -
dc.subject energy -
dc.subject enzyme activity -
dc.subject fermentation -
dc.subject hydrolysis -
dc.subject intertidal zone -
dc.subject nonhuman -
dc.subject nucleotide sequence -
dc.subject Psychrobacter -
dc.subject Bacteria (microorganisms) -
dc.subject Psychrobacter -
dc.subject Psychrobacter sp. -
dc.title Hydrolysis of triglycerides with cold-adapted lipase of Psychrobacter sp. S3 isolated from intertidal flat -
dc.type Article -
dc.citation.endPage 34 -
dc.citation.startPage 29 -
dc.citation.title Korean Journal of Microbiology and Biotechnology -
dc.citation.volume 33 -
dc.citation.number 1 -
dc.identifier.bibliographicCitation Korean Journal of Microbiology and Biotechnology, v.33, no.1, pp.29 - 34 -
dc.identifier.scopusid 2-s2.0-16444387023 -
dc.type.docType Article -
dc.identifier.kciid ART000950914 -
dc.description.journalClass 1 -
dc.subject.keywordPlus 4 nitrophenyl hexanoate -
dc.subject.keywordPlus agarose -
dc.subject.keywordPlus hexanoic acid derivative -
dc.subject.keywordPlus RNA 16S -
dc.subject.keywordPlus triacylglycerol -
dc.subject.keywordPlus triacylglycerol lipase -
dc.subject.keywordPlus tributyrin -
dc.subject.keywordPlus unclassified drug -
dc.subject.keywordPlus alkalinity -
dc.subject.keywordPlus article -
dc.subject.keywordPlus bacterial growth -
dc.subject.keywordPlus bacterial strain -
dc.subject.keywordPlus bacterium culture -
dc.subject.keywordPlus bacterium identification -
dc.subject.keywordPlus bacterium isolate -
dc.subject.keywordPlus bacterium isolation -
dc.subject.keywordPlus calculation -
dc.subject.keywordPlus cold acclimatization -
dc.subject.keywordPlus energy -
dc.subject.keywordPlus enzyme activity -
dc.subject.keywordPlus fermentation -
dc.subject.keywordPlus hydrolysis -
dc.subject.keywordPlus intertidal zone -
dc.subject.keywordPlus nonhuman -
dc.subject.keywordPlus nucleotide sequence -
dc.subject.keywordPlus Psychrobacter -
dc.subject.keywordPlus Bacteria (microorganisms) -
dc.subject.keywordPlus Psychrobacter -
dc.subject.keywordPlus Psychrobacter sp. -
dc.subject.keywordAuthor Lipase -
dc.subject.keywordAuthor Psychrobacter -
dc.subject.keywordAuthor Psychrophilic enzyme -
dc.subject.keywordAuthor Triglyceride -
dc.description.journalRegisteredClass scopus -
dc.description.journalRegisteredClass kci -
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Marine Resources Research Division > Marine Biotechnology Research Center > 1. Journal Articles
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