Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia coli ATCC 33456 SCIE SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Bae, WC | - |
dc.contributor.author | Lee, HK | - |
dc.contributor.author | Choe, YC | - |
dc.contributor.author | Jahng, DJ | - |
dc.contributor.author | Lee, SH | - |
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Lee, JH | - |
dc.contributor.author | Jeong, BC | - |
dc.date.accessioned | 2020-04-20T14:25:26Z | - |
dc.date.available | 2020-04-20T14:25:26Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2005-02 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/5103 | - |
dc.description.abstract | A soluble Cr(VI) reductase was purified from the cytoplasm of Escherichia coli ATCC 33456. The molecular mass was estimated to be 84 and 42 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis, respectively, indicating a dimeric structure. The pI was 4.66, and optimal enzyme activity was obtained at pH 6.5 and 37 degrees C. The most stable condition existed at pH 7.0. The purified enzyme used both NADPH and NADH as electron donors for Cr(VI) reduction, while NADPH was the better, conferring 61% higher activity than NADH. The K-m values for NADPH and NADH were determined to be 47.5 and 17.2 mu mol, and the V-max values 322 and 130.7 mu mol Cr(VI) min(-1)mg(-1) protein, respectively. The activity was strongly inhibited by N-ethylmalemide, Ag2+, Cd2+, Hg2+, and Zn2+. The antibody against the enzyme showed no immunological cross reaction with those of other Cr(VI) reducing strains. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.subject | PSEUDOMONAS-AMBIGUA G-1 | - |
dc.subject | HEXAVALENT CHROMIUM | - |
dc.subject | ENTEROBACTER-CLOACAE | - |
dc.subject | ENZYMATIC REDUCTION | - |
dc.subject | CHROMATE REDUCTASE | - |
dc.subject | MEMBRANE | - |
dc.subject | PROTEINS | - |
dc.subject | CULTURES | - |
dc.subject | PUTIDA | - |
dc.subject | STRAIN | - |
dc.title | Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia coli ATCC 33456 | - |
dc.type | Article | - |
dc.citation.endPage | 27 | - |
dc.citation.startPage | 21 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 43 | - |
dc.citation.number | 1 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.43, no.1, pp.21 - 27 | - |
dc.identifier.wosid | 000228323600004 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001099159 | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PSEUDOMONAS-AMBIGUA G-1 | - |
dc.subject.keywordPlus | HEXAVALENT CHROMIUM | - |
dc.subject.keywordPlus | ENTEROBACTER-CLOACAE | - |
dc.subject.keywordPlus | ENZYMATIC REDUCTION | - |
dc.subject.keywordPlus | CHROMATE REDUCTASE | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CULTURES | - |
dc.subject.keywordPlus | PUTIDA | - |
dc.subject.keywordPlus | STRAIN | - |
dc.subject.keywordAuthor | Cr(VI) reductase | - |
dc.subject.keywordAuthor | Escherichia coli ATCC 33456 | - |
dc.subject.keywordAuthor | purification | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Microbiology | - |