Kordiimonas gwangyangensis gen. nov., sp nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the 'Alphaproteobacteria'

Abstract

A marine bacterium, designated strain GW14-5(T), capable of degrading high-molecular-mass polycyclic aromatic hydrocarbons was isolated from the sediments of Gwangyang Bay, Republic of Korea, after enrichment culture for 2 years with a mixture of benzo[a]pyrene and pyrene. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate forms a phyletic lineage that is distinct from the seven known orders within the 'Alphaproteobacteria'. 16S rRNA gene sequence similarity of strain GW14-5(T) to all recognized bacterial species was not greater than 92 %. The dominant fatty acids of the isolate were i-17:1 (46(.)2 %), i-15:0 (15(.)1 %) and i-17:0 (12(.)6 %). The major respiratory quinone was MK-5, and the DNA G + C content was 39(.)3 mol%. Cells of strain GW14-5(T) were Gram-negative, motile, catalase-positive, oxidase-positive and weakly halophilic. Glucose, N-acetylglucosamine and maltose were utilized as sole carbon sources. The strain was positive for beta-glucosiclase activity. Optimal growth of strain GW14-5(T) was at pH 7(.)0 and 37-40 degrees C and required the presence of 2% (w/v) NaCl. On the basis of this evidence, strain GW14-5(T) represents a novel genus and species in the 'Alphaproteobacteria' for which the name Kordiimonas gwangyangensis gen. nov., sp. nov. is proposed. The novel order Kordiimonadales is proposed for the distinct phyletic line represented by the genus Kordiimonas. The type strain is GW14-5(T) (=KCCM 42021(T) = JCM 12864(T)).