Efficient isolation of intact RNA from the soft coral Scleronephthya gracillimum (Küenthal) for gene expression analyses KCI

Title
Efficient isolation of intact RNA from the soft coral Scleronephthya gracillimum (Küenthal) for gene expression analyses
Alternative Title
Efficient isolation of intact RNA from the soft coral Scleronephthya gracillimum (Küenthal) for gene expression analyses
Author(s)
우선옥; 염승식; 윤문근; 김사흥; 이종락; 김정하; 이택견
KIOST Author(s)
Yum, Seung Shic(염승식)Lee, Taek Kyun(이택견)
Publication Year
2005-12
Abstract
A rapid, simple and efficient method to extract RNA from the adult polyps of a soft coral, marine cnidarian, Scleronephthya gracillimum (Ku¨kenthal) ; was developed in this study. The highest yield and purity of RNA was obtained with the lysis solution containing 35 mM EDTA, 0.7 M LiCl, 7.0% SDS, and 200 mM Tris-Cl (pH 9.0). Approximately 40μg of total RNA was extracted from 200 mg of liquid nitrogen-pulverized polyp tissue. The ratio of absorbance at 260 nm and 280 nm ranged from 1.8 to 2.0. The results of the reverse transcription polymerase chain reaction (RTPCR) with β−actin gene specific primers and Northern blot analysis using the same gene probe revealed that the RNA extracted by our method had high quality, and was sufficient for subsequent molecular biological analyses. This method was effective for RNA extraction from other soft coral species which belong to the genus Dendronephthya.
ISSN
1976-8354
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4991
Bibliographic Citation
Animal Cells and Systems, v.9, no.4, pp.205 - 209, 2005
Publisher
한국통합생물학회
Keywords
Scleronephthya gracillimum; RNA isolation; northern blot; gene expression; soft coral
Type
Article
Language
English
Publisher
한국통합생물학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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