Comet assay를 이용한 갯지렁이 (Perinereis aibuhitensis)의 혈구세포에 대한 유전독성 평가

Abstract

In order to know whether polychaetes could be used as an appropriate organism for the detection ofgenotoxicity, DNA strand breaks were evaluated in blood cells of a nereidae worm (Perinereis aibuhitensis)exposed to various aquatic chemical pollutants (e.g. Cd, Pb, Pyrene, Benzo[a]pyrene). Hydrogen peroxideincreased DNA strand breaks up to the highest concentration (10M). Higher concentration than 0.1Mshowed a significantly more DNA damage than control. Cadmium and lead also showed higher DNA damagethan control, over 1.0 and 1g/L, respectively. In case of pyrene, DNA damage was detected even at 0.001study suggested that the polychaetous blood cells could be used effectively for screening genotoxic contami-nants in the environment.