Protein trans-splicing and characterization of a split family B-type DNA polymerase from the hyperthermophilic archaeal parasite Nanoarchaeum equitans SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Choi, JJ | - |
dc.contributor.author | Nam, KH | - |
dc.contributor.author | Min, B | - |
dc.contributor.author | Kim, SJ | - |
dc.contributor.author | Soll, D | - |
dc.contributor.author | Kwon, ST | - |
dc.date.accessioned | 2020-04-20T13:25:08Z | - |
dc.date.available | 2020-04-20T13:25:08Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2006-03-10 | - |
dc.identifier.issn | 0022-2836 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4905 | - |
dc.description.abstract | Nanoarchaeum equitans family B-type DNA polymerase (Neq DNA polymerase) is encoded by two separate genes, the large gene coding for the N-terminal part (Neq L) of Neq DNA polymerase and the small gene coding for the C-terminal part (Neq S), including a split mini-intein sequence. The two Neq DNA polymerase genes were cloned and expressed in Escherichia coli individually, together (for the Neq C), and as a genetically protein splicing-processed form (Neq P). The protein trans-spliced Neq C was obtained using the heating step at 80 degrees C after the co-expression of the two genes. The protein trans-splicing of the N-terminal and C-terminal parts of Neq DNA polymerase was examined in vitro using the purified Neq L and Neq S. The trans-splicing was influenced mainly by temperature, and occurred only at temperatures above 50 degrees C. The trans-splicing reaction was inhibited in the presence of zinc. Neq S has no catalytic activity and Neq L has lower 3'-5' exonuclease activity; whereas Neq C and Neq P have polymerase and 3'-5' exonuclease activities, indicating that both Neq L and Neq S are needed to form the active DNA polymerase that possesses higher proofreading activity. The genetically protein splicing-processed Neq P showed the same properties as the protein trans-spliced Neq C. Our results are the first evidence to show experimentally that natural protein trans-splicing occurs in an archaeal protein, a thermostable protein, and a family B-type DNA polymerase. (c) 2006 Elsevier Ltd. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | - |
dc.subject | THERMOAUTOTROPHICUM DELTA-H | - |
dc.subject | IN-VITRO | - |
dc.subject | PHYLUM NANOARCHAEOTA | - |
dc.subject | ZINC ION | - |
dc.subject | INTEIN | - |
dc.subject | GENE | - |
dc.subject | EXPRESSION | - |
dc.subject | SEQUENCE | - |
dc.subject | CLEAVAGE | - |
dc.subject | PCC6803 | - |
dc.title | Protein trans-splicing and characterization of a split family B-type DNA polymerase from the hyperthermophilic archaeal parasite Nanoarchaeum equitans | - |
dc.type | Article | - |
dc.citation.endPage | 1106 | - |
dc.citation.startPage | 1093 | - |
dc.citation.title | JOURNAL OF MOLECULAR BIOLOGY | - |
dc.citation.volume | 356 | - |
dc.citation.number | 5 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MOLECULAR BIOLOGY, v.356, no.5, pp.1093 - 1106 | - |
dc.identifier.doi | 10.1016/j.jmb.2005.12.036 | - |
dc.identifier.scopusid | 2-s2.0-32044468517 | - |
dc.identifier.wosid | 000235432400004 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | THERMOAUTOTROPHICUM DELTA-H | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PHYLUM NANOARCHAEOTA | - |
dc.subject.keywordPlus | ZINC ION | - |
dc.subject.keywordPlus | INTEIN | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | PCC6803 | - |
dc.subject.keywordAuthor | protein trans-splicing | - |
dc.subject.keywordAuthor | Nanoarchaeum equitans | - |
dc.subject.keywordAuthor | nanoarchaeon | - |
dc.subject.keywordAuthor | family B-type DNA polymerase | - |
dc.subject.keywordAuthor | biochemical characterization | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |