Novel pectin isolated from Spirulina maxima enhances the disease resistance and immune responses in zebrafish against Edwardsiella piscicida and Aeromonas hydrophila SCIE SCOPUS

DC Field Value Language
dc.contributor.author Edirisinghe, S. L. -
dc.contributor.author Dananjaya, S. H. S. -
dc.contributor.author Nikapitiya, Chamilani -
dc.contributor.author Liyanage, T. D. -
dc.contributor.author Lee, Kyoung-Ah -
dc.contributor.author Oh, Chulhong -
dc.contributor.author Kang, Hyung -
dc.contributor.author De Zoysa, Mahanama -
dc.date.accessioned 2020-04-16T07:05:26Z -
dc.date.available 2020-04-16T07:05:26Z -
dc.date.created 2020-02-04 -
dc.date.issued 2019-11 -
dc.identifier.issn 1050-4648 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/480 -
dc.description.abstract In this study, we demonstrate the enhanced disease resistance and positive immunomodulation of novel pectin isolated from Spirulina maxima (SmP) in zebrafish model. Zebrafish larvae exposed to SmP had significantly (p < 0.05) higher cumulative percent survival (CPS) at 25 (44.0%) and 50 mu g/mL (67.0%) against Edwardsieila piscicida compared to the control. However, upon Aeromonas hydrophila challenge, SmP exposed larvae at 50 mu g/mL had slightly higher CPS (33.3%) compared to control group (26.7%). SmP supplemented zebrafish exhibited the higher CPS against E. piscicida (93.3%) and A. hydrophila (60.0%) during the early stage of post-infection (< 18 hpi). qRT-PCR results demonstrated that exposing (larvae) and feeding (adults) of SmP, drive the modulation of a wide array of immune response genes. In SmP exposed larvae, up-regulation of the antimicrobial enzyme (lyz: 3.5-fold), mucin (muc5.1: 2.84, muc5.2: 2.11 and muc5.3: 2.40-fold), pro-inflammatory cytokines (il1 beta: 1.79-fold) and anti-oxidants (cat 2.87 and sod1: 1.82-fold) were identified. In SmP fed adult zebrafish (gut) showed > 2-fold induced pro-inflammatory cytokine (il1 beta) and chemokines (cxcl18b, ccl34a.4 and ccl34b.4). Overall results confirmed the positive modulation of innate immune responses in larval stage and it could be the main reason for developing disease resistance against E. piscicida and A. hydrophila. Thus, non-toxic, natural and biodegradable SmP could be considered as the potential immunomodulatory agent for sustainable aquaculture. -
dc.description.uri 1 -
dc.language English -
dc.publisher ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD -
dc.subject GENE-EXPRESSION -
dc.subject BETA-GLUCAN -
dc.subject GROWTH -
dc.title Novel pectin isolated from Spirulina maxima enhances the disease resistance and immune responses in zebrafish against Edwardsiella piscicida and Aeromonas hydrophila -
dc.type Article -
dc.citation.endPage 565 -
dc.citation.startPage 558 -
dc.citation.title FISH & SHELLFISH IMMUNOLOGY -
dc.citation.volume 94 -
dc.contributor.alternativeName 오철홍 -
dc.contributor.alternativeName 강도형 -
dc.identifier.bibliographicCitation FISH & SHELLFISH IMMUNOLOGY, v.94, pp.558 - 565 -
dc.identifier.doi 10.1016/j.fsi.2019.09.054 -
dc.identifier.scopusid 2-s2.0-85072562304 -
dc.identifier.wosid 000496892400059 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus GENE-EXPRESSION -
dc.subject.keywordPlus BETA-GLUCAN -
dc.subject.keywordPlus GROWTH -
dc.subject.keywordAuthor Aeromonas hydrophila -
dc.subject.keywordAuthor Mucin -
dc.subject.keywordAuthor Edwardsiella piscicida -
dc.subject.keywordAuthor Immunostimulant -
dc.subject.keywordAuthor Pectin -
dc.subject.keywordAuthor Spirulina maxima -
dc.relation.journalWebOfScienceCategory Fisheries -
dc.relation.journalWebOfScienceCategory Immunology -
dc.relation.journalWebOfScienceCategory Marine & Freshwater Biology -
dc.relation.journalWebOfScienceCategory Veterinary Sciences -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Fisheries -
dc.relation.journalResearchArea Immunology -
dc.relation.journalResearchArea Marine & Freshwater Biology -
dc.relation.journalResearchArea Veterinary Sciences -
Appears in Collections:
Jeju Research Institute > Jeju Bio Research Center > 1. Journal Articles
Jeju Research Institute > Tropical & Subtropical Research Center > 1. Journal Articles
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