Novel pectin isolated from Spirulina maxima enhances the disease resistance and immune responses in zebrafish against Edwardsiella piscicida and Aeromonas hydrophila SCIE SCOPUS

Cited 8 time in WEB OF SCIENCE Cited 10 time in Scopus
Title
Novel pectin isolated from Spirulina maxima enhances the disease resistance and immune responses in zebrafish against Edwardsiella piscicida and Aeromonas hydrophila
Author(s)
Edirisinghe, S. L.; Dananjaya, S. H. S.; Nikapitiya, Chamilani; Liyanage, T. D.; Lee, Kyoung-Ah; Oh, Chulhong; Kang, Hyung; De Zoysa, Mahanama
KIOST Author(s)
Oh, Chul Hong(오철홍)Kang, Do Hyung(강도형)
Publication Year
2019-11
Abstract
In this study, we demonstrate the enhanced disease resistance and positive immunomodulation of novel pectin isolated from Spirulina maxima (SmP) in zebrafish model. Zebrafish larvae exposed to SmP had significantly (p < 0.05) higher cumulative percent survival (CPS) at 25 (44.0%) and 50 mu g/mL (67.0%) against Edwardsieila piscicida compared to the control. However, upon Aeromonas hydrophila challenge, SmP exposed larvae at 50 mu g/mL had slightly higher CPS (33.3%) compared to control group (26.7%). SmP supplemented zebrafish exhibited the higher CPS against E. piscicida (93.3%) and A. hydrophila (60.0%) during the early stage of post-infection (< 18 hpi). qRT-PCR results demonstrated that exposing (larvae) and feeding (adults) of SmP, drive the modulation of a wide array of immune response genes. In SmP exposed larvae, up-regulation of the antimicrobial enzyme (lyz: 3.5-fold), mucin (muc5.1: 2.84, muc5.2: 2.11 and muc5.3: 2.40-fold), pro-inflammatory cytokines (il1 beta: 1.79-fold) and anti-oxidants (cat 2.87 and sod1: 1.82-fold) were identified. In SmP fed adult zebrafish (gut) showed > 2-fold induced pro-inflammatory cytokine (il1 beta) and chemokines (cxcl18b, ccl34a.4 and ccl34b.4). Overall results confirmed the positive modulation of innate immune responses in larval stage and it could be the main reason for developing disease resistance against E. piscicida and A. hydrophila. Thus, non-toxic, natural and biodegradable SmP could be considered as the potential immunomodulatory agent for sustainable aquaculture.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/480
DOI
10.1016/j.fsi.2019.09.054
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.94, pp.558 - 565, 2019
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
GENE-EXPRESSION; BETA-GLUCAN; GROWTH
Keywords
Aeromonas hydrophila; Mucin; Edwardsiella piscicida; Immunostimulant; Pectin; Spirulina maxima
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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