Screening of differentially expressed genes in diesel oil-exposed marine fish using DD-PCR
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Cited 3 time in
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Title
- Screening of differentially expressed genes in diesel oil-exposed marine fish using DD-PCR
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Author(s)
- Woo, Seonock; Yum, Seungshic; Yim, Un Hyuk; Lee, Taek-Kyun
- KIOST Author(s)
- Woo, Seon Ock(우선옥); Yum, Seung Shic(염승식); Yim, Un Hyuk(임운혁); Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 우선옥; 염승식; 임운혁; 이택견
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Publication Year
- 2006-12-31
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Abstract
- The exploration of genes which expressions are changed by exposure to ecotoxicants or pollutants can provide the important information about the reaction mechanisms in the body as well as adaptation to exterior stimulus or environmental changes. Also they can be developed as biomarkers for the detection of environmental pollution. Differential display polymerase chain reaction (DD-PCR) technique has been usefully used to hunt the clones which expressions are up-regulated or down-regulated by exterior changes and this study aimed to search for those clones in diesel oil-exposed rockfish (Sebastes schlegeli) using DD-PCR. The RNA isolated from liver of 20 ppb diesel oil-exposed rockfish was used for screening of the differentially displayed genes and total 44 differentially expressed genes (DEG) are detected then their nucleotide sequences were analyzed. The present data provided the general information about the effect of diesel oil contamination on marine organism and further more the primary step in development of new biomarkers for marine environmental pollution or ecotoxicological stresses.
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ISSN
- 1738-642X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4808
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Bibliographic Citation
- MOLECULAR & CELLULAR TOXICOLOGY, v.2, no.4, pp.251 - 256, 2006
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Publisher
- KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS
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Subject
- CONTROL PRIMER SYSTEM; PROTEIN-LEVELS; DISPLAY; HEMOCYTES; SEDIMENTS; CLONING
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Keywords
- Sebastes schlegeli; diesel; differential gene expression; biomarker
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Type
- Article
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Language
- English
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Document Type
- Article
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