Cloning, purification, and characterization of a new DNA polymerase from a hyperthermophilic archaeon, Thermococcus sp NA1 SCIE SCOPUS KCI
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yun Jae | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.contributor.author | Bae, Seung Seob | - |
dc.contributor.author | Jeon, Jeong Ho | - |
dc.contributor.author | Lim, Jae Kyu | - |
dc.contributor.author | Cho, Yona | - |
dc.contributor.author | Nam, Ki Hoon | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.contributor.author | Kim, Sang-Jin | - |
dc.contributor.author | Kwon, Suk-Tae | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.date.accessioned | 2020-04-20T11:55:17Z | - |
dc.date.available | 2020-04-20T11:55:17Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2007-07 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4677 | - |
dc.description.abstract | Genomic analysis of Thermococcus sp. TNA1 revealed the presence of a 3,927-base-pair (bp) family B-type DNA polymerase gene, TNA1_pol. TNA1_pol, without its intein, was overexpressed in Escherichia coli, purified using metal affinity chromatography, and characterized. TNA1_pol activity was optimal at pH 7.5 and 75 degrees C. TNA1_pol was highly thermostable, with a half-life of 3.5 h at 100 degrees C and 12.5 h at 95 degrees C. Polymerase chain reaction parameters of TNA1_pol such as error-rate, processivity, and extension rate were measured in comparison with rTaq, Pfu, and KOD DNA polymerases. TNA1_pol averaged one incorrect bp every 4.45 kilobases (kb), and had a processivity of 150 nucleotides (m) and an extension rate of 60 bases/s. Thus, TNA1_pol has a much faster elongation rate than Pfu DNA polymerase with 7-fold higher fidelity than that of rTaq. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | HIGH-FIDELITY | - |
dc.subject | PCR | - |
dc.subject | AMPLIFICATION | - |
dc.subject | PROCESSIVITY | - |
dc.subject | SEQUENCES | - |
dc.subject | COMPILATION | - |
dc.subject | EXPRESSION | - |
dc.subject | LITORALIS | - |
dc.subject | ENZYME | - |
dc.subject | KOD1 | - |
dc.title | Cloning, purification, and characterization of a new DNA polymerase from a hyperthermophilic archaeon, Thermococcus sp NA1 | - |
dc.type | Article | - |
dc.citation.endPage | 1097 | - |
dc.citation.startPage | 1090 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 17 | - |
dc.citation.number | 7 | - |
dc.contributor.alternativeName | 김윤재 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 배승섭 | - |
dc.contributor.alternativeName | 전정호 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 조요나 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.7, pp.1090 - 1097 | - |
dc.identifier.scopusid | 2-s2.0-34547987644 | - |
dc.identifier.wosid | 000248379500005 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HIGH-FIDELITY | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | PROCESSIVITY | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordPlus | COMPILATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | LITORALIS | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | KOD1 | - |
dc.subject.keywordAuthor | hyperthermophile | - |
dc.subject.keywordAuthor | characterization | - |
dc.subject.keywordAuthor | family B-type DNA polymerase | - |
dc.subject.keywordAuthor | processivity | - |
dc.subject.keywordAuthor | fidelity | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |