Critical factors to high thermostability of an alpha-amylase from hyperthermophilic archaeon Thermococcus onnurineus NA1 SCIE SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Lim, Jae Kyu | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.contributor.author | Kim, Yun Jae | - |
dc.contributor.author | Bae, Seung Seob | - |
dc.contributor.author | Jeon, Jeong Ho | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.date.accessioned | 2020-04-20T11:55:11Z | - |
dc.date.available | 2020-04-20T11:55:11Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2007-08 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4665 | - |
dc.description.abstract | Genomic analysis of a hyperthermophilic archaeon, Thermococcus onnurineus NA 1 [1], revealed the presence of an open reading frame consisting of 1,377 bp similar to alpha-amylases from Thermococcales, encoding a 458-residue polypeptide containing a putative 25-residue signal peptide. The mature form of the a-amylase was cloned and the recombinant enzyme was characterized. The optimum activity of the enzyme occurred at 80 degrees C and pH 5.5. The enzyme showed a liquefying activity, hydrolyzing maltooligosaccharides, amylopectin, and starch to produce mainly maltose (G2) to maltoheptaose (G7), but not pullulan and cyclodextrin. Surprisingly, the enzyme was not highly thermostable, with half-life (t(1/2)) values of 10 min at 90 degrees T, despite the high similarity to a-amylases from Pyrococcus. Factors affecting the thermostability were considered to enhance the thermostability. The presence of Ca2+ seemed to be critical, significantly changing t(1/2) at 90 degrees T to 153 min by the addition of 0.5 mM Ca2+. On the other hand, the thermostability was not enhanced by the addition of Zn2+ or other divalent metals, irrespective of the concentration. The mutagenetic study showed that the recovery of zinc-binding residues (His175 and Cys189) enhanced the thermostability, indicating that the residues involved in metal binding is very critical for the thermostability. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | PYROCOCCUS-FURIOSUS | - |
dc.subject | AMYLOLYTIC ENZYMES | - |
dc.subject | BIOCHEMICAL-CHARACTERIZATION | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | RECOMBINANT ENZYME | - |
dc.subject | EXPRESSION | - |
dc.subject | CLONING | - |
dc.subject | GENE | - |
dc.subject | PURIFICATION | - |
dc.subject | PROFUNDUS | - |
dc.title | Critical factors to high thermostability of an alpha-amylase from hyperthermophilic archaeon Thermococcus onnurineus NA1 | - |
dc.type | Article | - |
dc.citation.endPage | 1248 | - |
dc.citation.startPage | 1242 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 17 | - |
dc.citation.number | 8 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 김윤재 | - |
dc.contributor.alternativeName | 배승섭 | - |
dc.contributor.alternativeName | 전정호 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.8, pp.1242 - 1248 | - |
dc.identifier.scopusid | 2-s2.0-34548472445 | - |
dc.identifier.wosid | 000249111700003 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001078927 | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PYROCOCCUS-FURIOSUS | - |
dc.subject.keywordPlus | AMYLOLYTIC ENZYMES | - |
dc.subject.keywordPlus | BIOCHEMICAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | RECOMBINANT ENZYME | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | PROFUNDUS | - |
dc.subject.keywordAuthor | genomic analysis | - |
dc.subject.keywordAuthor | hyperthen-nophile | - |
dc.subject.keywordAuthor | cloning and expression | - |
dc.subject.keywordAuthor | alpha-amylase | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |