Growth characteristics and antioxidant properties of the benthic diatom Navicula incerta (Bacillariophyceae) from Jeju Island, Korea
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Title
- Growth characteristics and antioxidant properties of the benthic diatom Navicula incerta (Bacillariophyceae) from Jeju Island, Korea
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Author(s)
- Affan, A.; Karawita, R.; Jeon, Y.-J.; Lee, J.-B.
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Alternative Author(s)
- Abu Affan
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Publication Year
- 2007-08
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Abstract
- Benthic diatoms are a commonly used food source in shellfish aquaculture. Diatoms of the genus Navicula are the most abundant benthic diatoms occurring year-round on the coast of Jeju Island, Korea. We isolated an axenic strain of N. incerta Grunow; estimated its growth characteristics under 27 different combinations of temperature, salinity, and nutrients; and determined its biochemical composition and antioxidant activities. The maximum specific growth rate (μmax), defined as the increase in cell density per unit time, was 0.81-1.04·d-1, and the maximum cell density, 7.99 × 105 cells·mL-1, was reached at 0.88·d-1 μmax, 20°C, 30 psu salinity, and F/2 nutrient concentration on day 12 of the culture period. The approximate cellular composition was as follows: 7.0 ± 0.04% protein, 1.7 ± 0.28% lipid, 12.8 ± 0.85% carbohydrate, 68.4 ± 0.09% ash, and 10.1 ± 0.44% moisture. The antioxidant properties of N. incerta were determined for various extracts. The rates of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging for Neutrase and methanol extracts were 81.6% and 62.8%, respectively. Flavourzyme extract had a superoxide-scavenging rate of 57.7%. Kojizyme and Ultraflo extracts had nitric-oxide-scavenging rates of 42.2% and 40.6%, respectively, significantly higher than commercial antioxidants, such as α-tocopherol and butylated hydroxytoluene (BHT). The metal-chelating activities of the methanol, Neutrase, and Termamyl extracts were 68.5%, 45.2%, and 41.2%, respectively, four to six times higher than commercial antioxidants. The Termamyl extract showed the highest linoleic acid peroxidation inhibition, exceeding α-tocopherol and on par with BHT. © 2007 Phycological Society of America.
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ISSN
- 0022-3646
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4660
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DOI
- 10.1111/j.1529-8817.2007.00367.x
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Bibliographic Citation
- Journal of Phycology, v.43, no.4, pp.823 - 832, 2007
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Publisher
- Blackwell Publishing Inc.
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Keywords
- Antioxidant activities; Benthic diatoms; Growth characteristics; Mass culture; Navicula
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Type
- Article
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Language
- English
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Document Type
- Article
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