Application of pressurized fluid extraction technique in the gas chromatography-mass spectrometry determination of sterols from marine sediment samples SCIE SCOPUS

Cited 23 time in WEB OF SCIENCE Cited 23 time in Scopus
Title
Application of pressurized fluid extraction technique in the gas chromatography-mass spectrometry determination of sterols from marine sediment samples
Author(s)
Li, Donghoa; Dong, Meihua; Shim, Won Joon; Kannan, Narayanan
KIOST Author(s)
Shim, Won Joon(심원준)
Publication Year
2007-08-10
Abstract
In order to determine steroid compounds in GC/MS an analytical method using pressurized fluid extraction (PIE) was developed. While extracting in-house reference material (coastal sediment) typical recovery in PFE ranged from 80 to 120% (+/- 2.5-14.5) and the average extraction yield in PFE in comparison to conventional soxhlet extraction was 115%. In particular, the PFE showed higher extraction efficiency for C-29 and then sterols. Optimizing parameters such as temperature and pressure is critical in achieving this efficiency. Sterols in the sediment were derivatized with silyl reagent BSTFA in acetone for the final determination. A short column florisil cleanup offered the best separation of the GC/MS sensitive derivatives from co-contaminants. Thirty-three coastal sediment samples were analyzed using PFE and Soxhlet extraction methods. The results on extraction efficiency, silyl derivatization kinetics and purification efficiency demonstrated that PIE is far superior in extracting sterols from sediment samples. It is simple, fast, efficient and amenable for automation. (c) 2007 Elsevier B.V. All rights reserved.
ISSN
0021-9673
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4649
DOI
10.1016/j.chroma.2007.05.036
Bibliographic Citation
JOURNAL OF CHROMATOGRAPHY A, v.1160, no.1-2, pp.64 - 70, 2007
Publisher
ELSEVIER SCIENCE BV
Subject
ACCELERATED SOLVENT-EXTRACTION; SEWAGE POLLUTION; FECAL STEROLS; KYEONGGI BAY; QUANTITATIVE-DETERMINATION; AROMATIC-HYDROCARBONS; SOXHLET EXTRACTION; LIQUID EXTRACTION; COASTAL WATERS; SEA SEDIMENTS
Keywords
pressurized fluid extraction (PFE); soxhlet; sterols; sediment; Korea
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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