북쪽말똥성게, Strongylcentrotus intermedius를 이용한 생물검정 최적 발생조건
KCI
OTHER
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Title
- 북쪽말똥성게, Strongylcentrotus intermedius를 이용한 생물검정 최적 발생조건
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Alternative Title
- Optimal Conditions for the Embryonic Development of Sea Urchin, Strongylocentrotus intermeditus
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Author(s)
- 류태권; 성찬경; 한기명; 황인영; 이택견; 이창훈
- KIOST Author(s)
- Han, Gi Myung(한기명); Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 류태권; 한기명; 이택견
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Publication Year
- 2007-09
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Abstract
- Even though some standard developmental bioassay protocols for environmental assessment using seaurchins have already been described, there have not been many attempts to apply and modify these protocolsurchins commonly found in Korea. Prior to developing a new protocol, it is essential to know the optimal con-ditions for the bioassay procedures. We investigated the optimal conditions(temperature, salinity, and embryodensity) of the sea urchin Strongylocentrotus intermedius. The ideal temperature for developmental bioassay ofS. intermediuswas determined to be 15C and the time required for the embryo to become pluteus larva was 72S. intermediuswas between 30 to 32 psu,which is similar to the range found in the natural habitats of adult populations. The optimum density ofembryos at the beginning of bioassays was 100 embryos/mL. When the assays were carried out at higher densi-ties, the proportion of normally developed larvae decreased significantly.
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ISSN
- 2093-6400
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4640
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Bibliographic Citation
- 환경독성보건학회지, v.22, no.3, pp.211 - 218, 2007
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Publisher
- 환경독성보건학회
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Keywords
- Strongylocentrotus intermedius; temperature; salinity; density; embryonic development
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Type
- Article
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Language
- Korean
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