Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio SCIE SCOPUS

Cited 11 time in WEB OF SCIENCE Cited 13 time in Scopus
Title
Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio
Author(s)
Kang, B. J.; Jung, J. -H.; Lee, J. M.; Lim, S. -G.; Saito, H.; Kim, M. H.; Kim, Y. -J.; Saigusa, M.; Han, C. -H.
KIOST Author(s)
Jung, Jee Hyun(정지현)
Publication Year
2007-12
Abstract
We cloned and sequenced two vitellogenin (vg) cDNAs of the carp, Cyprinus carpio, using a cDNA library constructed from estradiol-17 beta (E-2)-treated livers. One was a novel, longer 5000 bp-long cDNA termed vg-B2 encoding 1624 amino acids in a single open reading frame. The other was a shorter cDNA (vg-B1), identical to that registered previously as carp vg cDNA in the international nucleotide sequence database. The deduced amino acid sequences of these two molecules were well-aligned with known vertebrate Vgs sharing common characteristics such as N-terminal lipovitellin I (LVI), phosvitin (PV) and C-terminal lipovitellin II (LVII). The novel Vg-B2 bore a highly conserved GL/ICG motif within the LVII region, in contrast to the shorter Vg-B1 that has a truncated C-tenninal and lacks the beta-component within the LVII region including the GL/ICG motif Both vg-B2 and vg-B1 genes were expressed in the livers of females and E-2-injected males. Western blot analysis using anti-Vg and anti-vitellin (Vn) antisera demonstrated that both Vg-B2 and Vg-B1 were detected as polypeptides with an estimated molecular mass of 180 kDa and 160 kDa, respectively, in the blood of females and E-2-injected males. The results suggest the potential utilization of these genes as sensitive xenoestrogenic markers. (c) 2007 Elsevier Inc. All rights reserved.
ISSN
1096-4959
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4613
DOI
10.1016/j.cbpb.2007.07.088
Bibliographic Citation
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v.148, no.4, pp.445 - 453, 2007
Publisher
ELSEVIER SCIENCE INC
Subject
ROCKFISH SEBASTES-SCHLEGELI; TROUT ONCORHYNCHUS-MYKISS; YOLK PROTEIN PRODUCTS; OOCYTE MATURATION; MULTIPLE VITELLOGENINS; MOLECULAR-CLONING; MARINE TELEOST; PELAGIC EGGS; FORMS; PURIFICATION
Keywords
biomarker carp; Cyprinus carpio; estradiol-17 beta (E-2); vitellogenin
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE INC
Related Researcher
Research Interests

Toxicology,Fish physiology,Marine environment risk assessment,독성학,어류생리학,해양환경 위해성평가

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