Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio
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Title
- Structural and expression analyses of two vitellogenin genes in the carp, Cyprinus carpio
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Author(s)
- Kang, B. J.; Jung, J. -H.; Lee, J. M.; Lim, S. -G.; Saito, H.; Kim, M. H.; Kim, Y. -J.; Saigusa, M.; Han, C. -H.
- KIOST Author(s)
- Jung, Jee Hyun(정지현)
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Alternative Author(s)
- 정지현
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Publication Year
- 2007-12
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Abstract
- We cloned and sequenced two vitellogenin (vg) cDNAs of the carp, Cyprinus carpio, using a cDNA library constructed from estradiol-17 beta (E-2)-treated livers. One was a novel, longer 5000 bp-long cDNA termed vg-B2 encoding 1624 amino acids in a single open reading frame. The other was a shorter cDNA (vg-B1), identical to that registered previously as carp vg cDNA in the international nucleotide sequence database. The deduced amino acid sequences of these two molecules were well-aligned with known vertebrate Vgs sharing common characteristics such as N-terminal lipovitellin I (LVI), phosvitin (PV) and C-terminal lipovitellin II (LVII). The novel Vg-B2 bore a highly conserved GL/ICG motif within the LVII region, in contrast to the shorter Vg-B1 that has a truncated C-tenninal and lacks the beta-component within the LVII region including the GL/ICG motif Both vg-B2 and vg-B1 genes were expressed in the livers of females and E-2-injected males. Western blot analysis using anti-Vg and anti-vitellin (Vn) antisera demonstrated that both Vg-B2 and Vg-B1 were detected as polypeptides with an estimated molecular mass of 180 kDa and 160 kDa, respectively, in the blood of females and E-2-injected males. The results suggest the potential utilization of these genes as sensitive xenoestrogenic markers. (c) 2007 Elsevier Inc. All rights reserved.
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ISSN
- 1096-4959
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4613
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DOI
- 10.1016/j.cbpb.2007.07.088
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Bibliographic Citation
- COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v.148, no.4, pp.445 - 453, 2007
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Publisher
- ELSEVIER SCIENCE INC
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Subject
- ROCKFISH SEBASTES-SCHLEGELI; TROUT ONCORHYNCHUS-MYKISS; YOLK PROTEIN PRODUCTS; OOCYTE MATURATION; MULTIPLE VITELLOGENINS; MOLECULAR-CLONING; MARINE TELEOST; PELAGIC EGGS; FORMS; PURIFICATION
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Keywords
- biomarker carp; Cyprinus carpio; estradiol-17 beta (E-2); vitellogenin
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Type
- Article
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Language
- English
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Document Type
- Article
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