Molecular parameters for assessing marine biotoxicity: Gene expressions of rockfish (Sebastes schlegeli) exposed to polycyclic aromatic hydrocarbons SCIE KCI OTHER

Cited 3 time in WEB OF SCIENCE
Title
Molecular parameters for assessing marine biotoxicity: Gene expressions of rockfish (Sebastes schlegeli) exposed to polycyclic aromatic hydrocarbons
Author(s)
Woo, Seonock; Yum, Seungshic; Park, Hong-Seog; Jung, Jee Hyun; Lee, Sukchan; Kim, So Jung; Lee, Taek Kyun
KIOST Author(s)
Yum, Seung Shic(염승식)Jung, Jee Hyun(정지현)Lee, Taek Kyun(이택견)
Publication Year
2007-12-31
Abstract
Environmental and anthropogenic changes affect the health and stability of marine ecosystem. In this study we aimed to identify molecular biomarkers for ecotoxicological pollutants risk assessment in the rockfish (Sebastes schlegeli). We designed primers based on conserved sequences by multiple alignments of target genes from related species, and cloned the partial cDNAs of cytochrome P450 (CYP1A1), glutathione S-transferase (GST), metallothionein (MT), superoxide dismutase (SOD), ubiquitin (UB), vitellogenin (VTG) and beta-actin by reverse transcription polymerase chain reaction (RT-PCR) from S. schlegeli. Northern blot results indicated that these six genes expressions were significantly induced by benzo[a]pyrene (BaP, 1 mu M) and that the level of each of their transcripts increased in BaP-exposed rockfish in a time-dependent manner. This study suggests that transcriptional changes in these six genes may be used for monitoring environmental exposure to polycyclic aromatic hydrocarbons (PAHs).
ISSN
1738-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4604
Bibliographic Citation
MOLECULAR & CELLULAR TOXICOLOGY, v.3, no.4, pp.267 - 272, 2007
Publisher
SPRINGER
Subject
CU/ZN-SUPEROXIDE-DISMUTASE; MYTILUS-EDULIS; INDUCIBLE ISOFORM; METALLOTHIONEIN; CONTAMINATION; DESTRUCTION; GLUTATHIONE; PROTEINS; HARBOR; CYP1A
Keywords
Sebastes schlegeli; environmental stress; benzo[a]pyrene; biomarkers; gene expression
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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