Molecular parameters for assessing marine biotoxicity: Gene expressions of rockfish (Sebastes schlegeli) exposed to polycyclic aromatic hydrocarbons
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Title
- Molecular parameters for assessing marine biotoxicity: Gene expressions of rockfish (Sebastes schlegeli) exposed to polycyclic aromatic hydrocarbons
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Author(s)
- Woo, Seonock; Yum, Seungshic; Park, Hong-Seog; Jung, Jee Hyun; Lee, Sukchan; Kim, So Jung; Lee, Taek Kyun
- KIOST Author(s)
- Yum, Seung Shic(염승식); Jung, Jee Hyun(정지현); Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 염승식; 정지현; 이택견
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Publication Year
- 2007-12-31
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Abstract
- Environmental and anthropogenic changes affect the health and stability of marine ecosystem. In this study we aimed to identify molecular biomarkers for ecotoxicological pollutants risk assessment in the rockfish (Sebastes schlegeli). We designed primers based on conserved sequences by multiple alignments of target genes from related species, and cloned the partial cDNAs of cytochrome P450 (CYP1A1), glutathione S-transferase (GST), metallothionein (MT), superoxide dismutase (SOD), ubiquitin (UB), vitellogenin (VTG) and beta-actin by reverse transcription polymerase chain reaction (RT-PCR) from S. schlegeli. Northern blot results indicated that these six genes expressions were significantly induced by benzo[a]pyrene (BaP, 1 mu M) and that the level of each of their transcripts increased in BaP-exposed rockfish in a time-dependent manner. This study suggests that transcriptional changes in these six genes may be used for monitoring environmental exposure to polycyclic aromatic hydrocarbons (PAHs).
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ISSN
- 1738-642X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4604
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Bibliographic Citation
- MOLECULAR & CELLULAR TOXICOLOGY, v.3, no.4, pp.267 - 272, 2007
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Publisher
- SPRINGER
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Subject
- CU/ZN-SUPEROXIDE-DISMUTASE; MYTILUS-EDULIS; INDUCIBLE ISOFORM; METALLOTHIONEIN; CONTAMINATION; DESTRUCTION; GLUTATHIONE; PROTEINS; HARBOR; CYP1A
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Keywords
- Sebastes schlegeli; environmental stress; benzo[a]pyrene; biomarkers; gene expression
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Type
- Article
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Language
- English
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Document Type
- Article
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