BiochemicalCharacterizationandApplicationsofRecombinantGaa16BderivedfromGilvimarinusagarilyticusJEA5:APromisingToolforNeoagarooligosaccharideProduction
- Title
- BiochemicalCharacterizationandApplicationsofRecombinantGaa16BderivedfromGilvimarinusagarilyticusJEA5:APromisingToolforNeoagarooligosaccharideProduction
- KIOST Author(s)
- Shalini, Upekha Sithumini(Sithumini); Lee, Young Deuk(이영득); Jo, Eunyoung(조은영); Oh, Chul Hong(오철홍)
- Alternative Author(s)
- Sithumini; 이영득; 조은영; 오철홍
- Publication Year
- 2023-10-03
- Abstract
- Agar,acompoundfoundinseveralspeciesofredseaweed,iswidelyemployedindiverseindustries.Notably,oligosaccharidesderivedfromagarpossessvaluablebiologicalandtherapeuticproperties.Inthisresearch,gaa16bgeneobtainedfromthemarinebacteriumGilvimarinusagarilyticusJEA5wasdiscoveredtoencodeanovelβ-agaroseenzyme.Thegaa16bgenewasclonedandexpressedasarecombinantproteininE.coliBL21(DE3).Theresultingprotein,taggedwithmaltose-bindingprotein(MBP),waspurifiedandanalyzedtoexploreitsbiochemicalpropertiesandhydrolyticpatterns.Furthermore,ahyaluronidaseinhibitoryassaywasconductedtoevaluatethepotentialofthepartiallyhydrolyzedproduct(PHP)ofrecombinantGaa16B(rGaa16B)forcosmeticapplications.
- Bibliographic Citation
- 13th Asia-Pacific Marine Biotechnology Conference, 2023
- Publisher
- Flinders University
- Type
- Conference
- Language
- English
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- There are no files associated with this item.
