Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria
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Title
- Genomic potential and physiological characteristics of C1 metabolism in novel acetogenic bacteria
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Author(s)
- Yu, Ji Hyun; Park, Mi-Jeong; Lee, Joungmin; Kwon, Soo Jae; Lim, Jae Kyu; Lee, Hyun Sook; Kang, Sung Gyun; Lee, Jung Hyun; Kwon, Kae Kyoung; Kim, Yun Jae
- KIOST Author(s)
- Yu, Ji Hyun(유지현); Park, Mi-Jeong(박미정); Lee, Joungmin(이종민); Kwon, Soo Jae(권수재); Lim, Jae Kyu(임재규); Lee, Hyun Sook(이현숙); Kang, Sung Gyun(강성균); Lee, Jung Hyun(이정현); Kwon, Kae Kyoung(권개경); Kim, Yun Jae(김윤재)
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Alternative Author(s)
- 유지현; 박미정; 이종민; 권수재; 임재규; 이현숙; 강성균; 이정현; 권개경; 김윤재
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Publication Year
- 2023-10
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Abstract
- Acetogenic bacteria can utilize C1 compounds, such as carbon monoxide (CO), formate, and methanol, via the Wood-Ljungdahl pathway (WLP) to produce biofuels and biochemicals. Two novel acetogenic bacteria of the family Eubacteriaceae ES2 and ES3 were isolated from Eulsukdo, a delta island in South Korea. We conducted whole genome sequencing of the ES strains and comparative genome analysis on the core clusters of WLP with Acetobacterium woodii DSM1030T and Eubacterium limosum ATCC8486T. The methyl-branch cluster included a formate transporter and duplicates or triplicates copies of the fhs gene, which encodes formyl-tetrahydrofolate synthetase. The formate dehydrogenase cluster did not include the hydrogenase gene, which might be replaced by a functional complex with a separate electron bifurcating hydrogenase (HytABCDE). Additionally, duplicated copies of the acsB gene, encoding acetyl-CoA synthase, are located within or close to the carbonyl-branch cluster. The serum bottle culture showed that ES strains can utilize a diverse range of C1 compounds, including CO, formate, and methanol, as well as CO2. Notably, ES2 exhibited remarkable resistance to high concentrations of C1 substrates, such as 100% CO (200 kPa), 700 mM formate, and 500 mM methanol. Moreover, ES2 demonstrated remarkable growth rates under 50% CO (0.45 h−1) and 200 mM formate (0.34 h−1). These growth rates are comparable to or surpassing those previously reported in other acetogenic bacteria. Our study introduces novel acetogenic ES strains and describes their genetic and physiological characteristics, which can be utilized in C1-based biomanufacturing.
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ISSN
- 1664-302X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/44694
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DOI
- 10.3389/fmicb.2023.1279544
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Bibliographic Citation
- Frontiers in Microbiology, v.14, 2023
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Publisher
- Frontiers Media S.A.
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Keywords
- acetogenic bacteria; C1 compounds; formate; carbon monoxide; methanol; ES strains
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Type
- Article
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Language
- English
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Document Type
- Article
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