Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium SCIE KCI OTHER

Cited 2 time in WEB OF SCIENCE
Title
Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium
Author(s)
Woo, Seonock; Son, Sung Hee; Park, Hong-Seog; Vulpe, Chris D.; Ryu, Jae-Chun; Yum, Seungshic
KIOST Author(s)
Woo, Seon Ock(우선옥)Yum, Seung Shic(염승식)
Publication Year
2008-12-31
Abstract
To screen the differentially expressed genes in cadmuim-exposed marine medaka fish (Oryzias javanicus), a candidate marine test fish for ecological toxicity, the differential display polymerase chain reaction (DD-PCR) was carried out, since the genome-wide gene expression data are not available in this fish species yet. A total of 35 clones were isolated from cadmium-exposed fish and their nucleotide sequences were analyzed. The differentially expressed gene candidates were categorized to response to stimulus (3); ion binding (3); DNA binding (1); protein binding (6); carbohydrate binding (1); metabolic process (4); biological regulation (3); cellular process (2); protein synthesis (2); catalytic activity (2); sense of sight (1); immune (1); neurohormone (1); signaling activity (1); electron carrier activity (1) and others (3). For real-time quantitative RT-PCR, we selected catalase, glucose-6-phosphate dehydrogenase, heat shock protein 70, and metallothionein and confirmed that cadmium exposure enhanced induction of these four genes.
ISSN
1738-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4412
Bibliographic Citation
MOLECULAR & CELLULAR TOXICOLOGY, v.4, no.4, pp.293 - 299, 2008
Publisher
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
Subject
TROUT SALMO-TRUTTA; OXIDATIVE STRESS; JAVA-MEDAKA; ANTIOXIDANT ENZYMES; MESSENGER-RNA; INDUCTION; METALLOTHIONEIN; BIOMARKERS; DISPLAY; CELLS
Keywords
Oryzias javanicus; Cadmium; Differential gene expression; Biomarker; Environmental stress
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
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