Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium SCIE KCI OTHER

Title
Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium
Author(s)
Woo, Seonock; Son, Sung Hee; Park, Hong-Seog; Vulpe, Chris D.; Ryu, Jae-Chun; Yum, Seungshic
KIOST Author(s)
Yum, Seung Shic(염승식)
Publication Year
2008-12-31
Abstract
To screen the differentially expressed genes in cadmuim-exposed marine medaka fish (Oryzias javanicus), a candidate marine test fish for ecological toxicity, the differential display polymerase chain reaction (DD-PCR) was carried out, since the genome-wide gene expression data are not available in this fish species yet. A total of 35 clones were isolated from cadmium-exposed fish and their nucleotide sequences were analyzed. The differentially expressed gene candidates were categorized to response to stimulus (3); ion binding (3); DNA binding (1); protein binding (6); carbohydrate binding (1); metabolic process (4); biological regulation (3); cellular process (2); protein synthesis (2); catalytic activity (2); sense of sight (1); immune (1); neurohormone (1); signaling activity (1); electron carrier activity (1) and others (3). For real-time quantitative RT-PCR, we selected catalase, glucose-6-phosphate dehydrogenase, heat shock protein 70, and metallothionein and confirmed that cadmium exposure enhanced induction of these four genes.
ISSN
1738-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4412
Bibliographic Citation
MOLECULAR & CELLULAR TOXICOLOGY, v.4, no.4, pp.293 - 299, 2008
Publisher
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
Subject
TROUT SALMO-TRUTTA; OXIDATIVE STRESS; JAVA-MEDAKA; ANTIOXIDANT ENZYMES; MESSENGER-RNA; INDUCTION; METALLOTHIONEIN; BIOMARKERS; DISPLAY; CELLS
Keywords
Oryzias javanicus; Cadmium; Differential gene expression; Biomarker; Environmental stress
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

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