Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium
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Title
- Differentially Expressed Genes in Marine Medaka Fish (Oryzias javanicus) Exposed to Cadmium
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Author(s)
- Woo, Seonock; Son, Sung Hee; Park, Hong-Seog; Vulpe, Chris D.; Ryu, Jae-Chun; Yum, Seungshic
- KIOST Author(s)
- Woo, Seon Ock(우선옥); Yum, Seung Shic(염승식)
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Alternative Author(s)
- 우선옥; 손성희; 염승식
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Publication Year
- 2008-12-31
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Abstract
- To screen the differentially expressed genes in cadmuim-exposed marine medaka fish (Oryzias javanicus), a candidate marine test fish for ecological toxicity, the differential display polymerase chain reaction (DD-PCR) was carried out, since the genome-wide gene expression data are not available in this fish species yet. A total of 35 clones were isolated from cadmium-exposed fish and their nucleotide sequences were analyzed. The differentially expressed gene candidates were categorized to response to stimulus (3); ion binding (3); DNA binding (1); protein binding (6); carbohydrate binding (1); metabolic process (4); biological regulation (3); cellular process (2); protein synthesis (2); catalytic activity (2); sense of sight (1); immune (1); neurohormone (1); signaling activity (1); electron carrier activity (1) and others (3). For real-time quantitative RT-PCR, we selected catalase, glucose-6-phosphate dehydrogenase, heat shock protein 70, and metallothionein and confirmed that cadmium exposure enhanced induction of these four genes.
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ISSN
- 1738-642X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4412
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Bibliographic Citation
- MOLECULAR & CELLULAR TOXICOLOGY, v.4, no.4, pp.293 - 299, 2008
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Publisher
- KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
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Subject
- TROUT SALMO-TRUTTA; OXIDATIVE STRESS; JAVA-MEDAKA; ANTIOXIDANT ENZYMES; MESSENGER-RNA; INDUCTION; METALLOTHIONEIN; BIOMARKERS; DISPLAY; CELLS
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Keywords
- Oryzias javanicus; Cadmium; Differential gene expression; Biomarker; Environmental stress
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Type
- Article
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Language
- English
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Document Type
- Article
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