Thermococcus argininiproducens sp. nov., an arginine biosynthesis archaeal species isolated from the Central Indian Ocean ridge
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Title
- Thermococcus argininiproducens sp. nov., an arginine biosynthesis archaeal species isolated from the Central Indian Ocean ridge
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Author(s)
- Park, Yeong Jun; Lim, Jae Kyu; Kim, Yun Jae; Yang, Sung Hyun; Lee, Hyun Sook; Kang, Sung Gyun; Lee, Jung Hyun; Yang, Youngik; Kwon, Kae Kyoung
- KIOST Author(s)
- Park, Yeong Jun(박영준); Lim, Jae Kyu(임재규); Kim, Yun Jae(김윤재); Yang, Sung Hyun(양성현); Lee, Hyun Sook(이현숙); Kang, Sung Gyun(강성균); Lee, Jung Hyun(이정현); Kwon, Kae Kyoung(권개경)
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Alternative Author(s)
- 박영준; 임재규; 김윤재; 양성현; 이현숙; 강성균; 이정현; 권개경
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Publication Year
- 2023-04
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Abstract
- A strictly anaerobic hyperthermophilic archaeon, designated strain IOH2T, was isolated from a deep-sea hydrothermal vent (Onnuri vent field) area on the Central Indian Ocean Ridge. Strain IOH2T showed high 16S rRNA gene sequence similarity to Thermococcus sibiricus MM 739T (99.42 %), Thermococcus alcaliphilus DSM 10322T (99.28 %), Thermococcus aegaeus P5T (99.21 %), Thermococcus litoralis DSM 5473T (99.13 %), ‘Thermococcus bergensis’ T7324T (99.13 %), Thermococcus aggregans TYT (98.92 %) and Thermococcus prieurii Bio-pl-0405IT2T (98.01 %), with all other strains showing lower than 98 % similarity. The average nucleotide identity and in silico DNA–DNA hybridization values were highest between strain IOH2T and T. sibiricus MM 739T (79.33 and 15.00 %, respectively); these values are much lower than the species delineation cut-offs. Cells of strain IOH2T were coccoid, 1.0–1.2 µm in diameter and had no flagella. Growth ranges were 60–85 °C (optimum at 80 °C), pH 4.5–8.5 (optimum at pH 6.3) and 2.0–6.0 % (optimum at 4.0 %) NaCl. Growth of strain IOH2T was enhanced by starch, glucose, maltodextrin and pyruvate as a carbon source, and elemental sulphur as an electron acceptor. Through genome analysis of strain IOH2T, arginine biosynthesis related genes were predicted, and growth of strain IOH2T without arginine was confirmed. The genome of strain IOH2T was assembled as a circular chromosome of 1 946 249 bp and predicted 2096 genes. The DNA G+C content was 39.44 mol%. Based on the results of physiological and phylogenetic analyses, Thermococcus argininiproducens sp. nov. is proposed with type strain IOH2T (=MCCC 4K00089T=KCTC 25190T).
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ISSN
- 1466-5026
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/44008
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DOI
- 10.1099/ijsem.0.005760
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Bibliographic Citation
- International Journal of Systematic and Evolutionary Microbiology, v.73, no.4, 2023
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Publisher
- Society for General Microbiology
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Keywords
- arginine biosynthesis; Central Indian Ocean Ridge; hydrothermal vent; Onnuri vent field; thermococcus
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Type
- Article
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Language
- English
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Document Type
- Article
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