Nisaea acidiphila sp. nov., isolated from a marine algal debris, emended description of the genus Nisaea Urios et al. 2008, and the emendation of Thalassobaculum salexigens as Thalassobaculum litoreum subsp. salexigens comb. nov.

Abstract

A Gram-negative, facultatively anaerobic, rod-shaped (1.4 ± 0.46 μm × 0.53 ± 0.17 μm) and motile marine bacterium designated as MEBiC11861T was isolated from a marine algal debris collected from Kosrae, Federation State of Micronesia (162°57’23.1"E, 5°21’13.0"N). Based on the 16S rRNA gene sequence analysis strain MEBiC11861T identified as a novel species in the genus Nisaea, it showed high similarity to members of the genus Nisaea (97.0–98.4%). Strain MEBiC11861T was growing at 10–42℃ (optimum 26–29℃), at pH 4.0–8.5 (optimum pH 5.0) and with 0–10% (optimum 0.5%) NaCl. The C12:0 (5.6%), C16:0 (29.0%), C12:0 3-OH (4.3%), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c; 9.9%), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c; 31.2%), and C19:0 cyclo ω8c (10.6%) were determined to be as predominant cellular fatty acids. The DNA G + C content is 63.0 mol%. The major respiratory quinone is Q-10. Several phenotypic characteristics such as utilization of gluconate, malate, adipate, arabinose etc., DNA G + C ratio, cellular fatty acids composition, and growth range of pH and salinity differentiate strain MEBiC11861T from members of the genus Nisaea. On the basis of this polyphasic taxonomic data, strain MEBiC11861T should be classified as a novel species in the genus Nisaea and it is proposed as Nisaea acidiphila sp. nov. The type strain is MEBiC11861T (= KCCM 43219T = JCM 31589T). Emended description of the genus Nisaea Urios et al. 2008 and emendation of Thalassobaculum salexigens were also given.