Potential antioxidant effect from the viscera peptides of turbo cornutus

Abstract

The horned turban sea snail, Turbo Cornutus, is found along the intertidal and basalt coastlines of Jeju Island. T. cornutus eats seaweed, which is composed of a variety of antioxidants. This study identified potential antioxidant properties from T. cornutus viscera parts. Diverse hydrolysates were evaluated for their hydrogen peroxide (H2O2) scavenging activities. T. cornutus viscera protamex-assisted hydrolysates (TVPH) were purified by gel filtration chromatography (GFC), and potential antioxidant properties were analyzed for their amino acid sequences and its peroxidase inhibition effects by in silico analysis. According to the results, T. cornutus viscera parts are composed of many protein contents with each over 50%. Among the hydrolysates, TVPH possessed the highest H2O2 scavenging activity. Also, TVPH-GFC-3 significantly decreased intracellular reactive oxygen species (ROS) levels and increased cell viability in H2O2-treated HepG2 cells without noncytotoxicity. TVPH-GFC-3 comprises nine low molecular bioactive peptides (ELR, VGPQ, TDY, ALPHA, PAH, VDY, WSDK, VFSP, and FAPQY). Notably, in silico analysis predicted that these bioactive peptides dock to the active site of myeloperoxidase (MPO) with more stable binding energy than 4-aminobenzoic acid hydrazide (4-ABH), an inhibitor of MPO. Altogether, our findings demonstrated that T. cornutus viscera have potential antioxidant properties that can be used as high value-added ingredients.