Cloning and characterization of an epoxide hydrolase from Novosphingobium aromaticivorans SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Woo, Jung-Hee | - |
dc.contributor.author | Kang, Ji-Hyun | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.contributor.author | Hwang, Young-Ok | - |
dc.contributor.author | Kim, Sang-Jin | - |
dc.date.accessioned | 2020-04-20T09:55:07Z | - |
dc.date.available | 2020-04-20T09:55:07Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4312 | - |
dc.description.abstract | A gene encoding a putative epoxide hydrolase (EHase) was identified by analyzing an open reading frame of the genome sequence of Novosphingobium aromaticivorans, retaining the conserved catalytic residues such as the catalytic triad (Asp177, Glu328, and His355) and the oxyanion hole. The enantioselective EHase gene (neh) was cloned, and the recombinant EHase could be purified to apparent homogeneity by one step of metal affinity chromatography and further characterized. The purified N. aromaticivorans enantioselective epoxide hydrolase (NEH) showed enantioselective hydrolysis toward styrene oxide, glycidyl phenyl ether, epoxybutane, and epichlorohydrin. The optimal EHase activity toward styrene oxide occurred at pH 6.5 and 45A degrees C. The purified NEH could preferentially hydrolyze (R)-styrene oxide with enantiomeric excess of more than 99% and 11.7% yield after 20-min incubation at an optimal condition. The enantioselective hydrolysis of styrene oxide was also confirmed by the analysis of the vicinal diol, 1-phenyl-1,2-ethanediol. The hydrolyzing rates of the purified NEH toward epoxide substrates were not affected by as high as 100 mM racemic styrene oxide. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | AGROBACTERIUM-RADIOBACTER AD1 | - |
dc.subject | CATALYTIC MECHANISM | - |
dc.subject | SEQUENCE ALIGNMENT | - |
dc.subject | MARINE BACTERIUM | - |
dc.subject | ENCODING GENE | - |
dc.subject | EXPRESSION | - |
dc.subject | ENANTIOSELECTIVITY | - |
dc.subject | ERYTHROBACTER | - |
dc.subject | HYDROLYSIS | - |
dc.subject | ESTERASE | - |
dc.title | Cloning and characterization of an epoxide hydrolase from Novosphingobium aromaticivorans | - |
dc.type | Article | - |
dc.citation.endPage | 881 | - |
dc.citation.startPage | 873 | - |
dc.citation.title | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 82 | - |
dc.citation.number | 5 | - |
dc.contributor.alternativeName | 우정희 | - |
dc.contributor.alternativeName | 강지현 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 황영옥 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.identifier.bibliographicCitation | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.82, no.5, pp.873 - 881 | - |
dc.identifier.doi | 10.1007/s00253-008-1791-9 | - |
dc.identifier.scopusid | 2-s2.0-62949131374 | - |
dc.identifier.wosid | 000264460500009 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | AGROBACTERIUM-RADIOBACTER AD1 | - |
dc.subject.keywordPlus | CATALYTIC MECHANISM | - |
dc.subject.keywordPlus | SEQUENCE ALIGNMENT | - |
dc.subject.keywordPlus | MARINE BACTERIUM | - |
dc.subject.keywordPlus | ENCODING GENE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ENANTIOSELECTIVITY | - |
dc.subject.keywordPlus | ERYTHROBACTER | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | ESTERASE | - |
dc.subject.keywordAuthor | Epoxide hydrolase | - |
dc.subject.keywordAuthor | Enantioselectivity | - |
dc.subject.keywordAuthor | Diol configuration | - |
dc.subject.keywordAuthor | Genome | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |