Stimulatory effects of wavelength-dependent photobiomodulation on proliferation and angiogenesis of colorectal cancer SCIE SCOPUS

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Title
Stimulatory effects of wavelength-dependent photobiomodulation on proliferation and angiogenesis of colorectal cancer
Author(s)
Kim, Hyejin; Kim, Yeongeun; Kim, Tae-Hee; Heo, Seong Yeong; Jung, Won-Kyo; Kang, Hyun Wook
KIOST Author(s)
Heo, Seong Yeong(허성영)
Alternative Author(s)
허성영
Publication Year
2022-09
Abstract
In recent decades, the laser treatment of cancer has been introduced as a promising treatment option. Because of the maldistribution of optical energy and an ambiguous boundary between the normal and tumor tissues, laser irradiation can stimulate residual cancer cells, leading to a cancer regrowth. As photobiomodulation (PBM) is involved in an extensive range of cellular responses, profound comprehension of photo-stimulated mechanisms against the cancer cells is required to establish a safety margin for PBM. Therefore, we aimed to identify the stimulant effects of PBM at various wavelengths against the tumor cells to establish a safety margin for the laser treatment. CT26 murine colon cancer cells were exposed to either 405 (BL), 635 (VIS), or 808 (NIR) nm laser lights at the fluences of 0, 10, 30, and 50 J/cm2. In addition, CT26 tumor-bearing mice were irradiated with BL, VIS, or NIR at a fluence of 30 J/cm2. Both the proliferation and angiogenesis potential of the CT26 cells and tumors were evaluated using the MTT assay, western blot, and immunohistochemistry (IHC) staining analyses. Although cell viability was not statistically significant, BL significantly induced p-ERK upregulation in the CT26 cells, indicating that PBM with BL can stimulate proliferation. In vivo tests showed that the NIR group exhibited the maximum relative tumor volume, and BL yielded a slight increase compared to the control. In the IHC staining and western blot analyses, both BL and NIR increased the expression of EGFR, VEGF, MMP-9, and HIF-1α, which are related to the proliferation and angiogenesis-related factors. Further investigations will be pursued to clarify the molecular pathways that depend on the cancer cell types and laser wavelengths for the establishment of safety guidelines in clinical environments. © 2022 Elsevier B.V.
ISSN
1011-1344
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/43121
DOI
10.1016/j.jphotobiol.2022.112527
Bibliographic Citation
Journal of Photochemistry and Photobiology B: Biology, v.234, 2022
Publisher
Elsevier B.V.
Keywords
Angiogenesis; Cancer stimulation; Photobiomodulation; Proliferation; Wavelength effect
Type
Article
Language
English
Document Type
Article
Publisher
Elsevier B.V.
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