CO 내성을 갖는 Clostridium sp. AWRP 돌연변이주의 분리 및 이를 이용한 합성가스로부터의 에탄올 생산 연구 KCI

Title
CO 내성을 갖는 Clostridium sp. AWRP 돌연변이주의 분리 및 이를 이용한 합성가스로부터의 에탄올 생산 연구
Alternative Title
Enhanced Alcohol Production from Synthesis Gas Using a CO-resistant Mutant of Clostridium sp. AWRP
Author(s)
권수재; 이종민; 이현숙
KIOST Author(s)
Kwon, Soo Jae(권수재)Lee, Joung Min(이종민)Lee, Hyun Sook(이현숙)
Publication Year
2019-12
Abstract
In this study, the carbon monoxide (CO)-fermenting acetogen, Clostridium sp. AWRP was subjected to chemical mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to generate a CO-resistant mutant. Among the 26 colonies obtained, the highest alcohol production was observed in one isolate, named C1. Compared to the wild-type strain, the C1 strain exhibited 1.5- and 3.4-fold higher CO consumption rate and alcohol selectivity, respectively. The total CO consumption of strain C1 could be further enhanced by increasing the content of metal ions, such as nickel and iron. The highest ethanol titer (5.7 g/l) was achieved by 5-fold increase in the iron concentration.
ISSN
1598-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/431
Bibliographic Citation
한국미생물·생명공학회지, v.47, no.4, pp.581 - 584, 2019
Publisher
한국미생물·생명공학회
Keywords
Acetogen; synthesis gas; random mutagenesis
Type
Article
Language
Korean
Publisher
한국미생물·생명공학회
Related Researcher
Research Interests

Metabolic engineering,Marine microbiology,대사공학,해양미생물

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