Flow cytometry studies on the populations and immune parameters of the hemocytes of the Suminoe oyster, Crassostrea ariakensis SCIE SCOPUS

Cited 85 time in WEB OF SCIENCE Cited 91 time in Scopus
Title
Flow cytometry studies on the populations and immune parameters of the hemocytes of the Suminoe oyster, Crassostrea ariakensis
Author(s)
Donaghy, Ludovic; Kim, Bong-Kyu; Hong, Hyun-Ki; Park, Heung-Sik; Choi, Kwang-Sik
KIOST Author(s)
Park, Heung Sik(박흥식)
Publication Year
2009-08
Abstract
The Suminoe oyster Crassostrea ariakensis has been attempted to be introduced in the Chesapeake Bay, USA, as an alternative to the eastern oyster Crassostrea virginica. Commercial production of Suminoe oysters is currently restricted due to the incomplete understanding of their biological, physiological and immunological nature. Accordingly, understanding immune system of C. ariakensis is crucial to prevent disease associated mortality and subsequent management of the Suminoe oyster. We investigated immunological activities and morphology of hemocytes of the Suminoe oyster using flow cytometry and light microscopy. Three types of hemocytes were identified in the hemolymph including hyalinocyte, granulocyte and blast-like cells. Hyalinocytes were the largest cells and the most abundant, while granulocytes were intermediate-size cell containing numerous granules in the cytoplasm. Blast-like cells were the smallest and least numerous. Flow cytometry revealed that the granulocytes are most active in the cell phagocytosis and spontaneous reactive oxygen species (ROS) production. The hyalinocytes also showed a certain level of the phagocytosis and oxidative activity but in a lesser extent than the granulocytes. In contrasts the blast-like cells did not show any phagocytosis or oxidative activity. The flow cytometry used in this study confirmed that as observed from other marine bivalves, the granulocytes are the main hemocytes involved in the cellular defence in the Suminoe oyster. (C) 2009 Elsevier Ltd. All rights reserved.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4277
DOI
10.1016/j.fsi.2009.05.010
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.27, no.2, pp.296 - 301, 2009
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
EUROPEAN FLAT OYSTER; MERCENARIA-MERCENARIA HEMOCYTES; DECUSSATUS MOLLUSCA-BIVALVIA; HAPLOSPORIDIUM-NELSONI MSX; CLAM TAPES-PHILIPPINARUM; RUDITAPES-PHILIPPINARUM; CHESAPEAKE BAY; OSTREA-EDULIS; MANILA CLAM; REACTIVE OXYGEN
Keywords
Crassostrea ariakensis; Suminoe oyster; Flow cytometry; Granulocytes; Hyalinocytes; Blast-like cells; Phagocytosis; Reactive oxygen species
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse