Flow cytometry studies on the populations and immune parameters of the hemocytes of the Suminoe oyster, Crassostrea ariakensis
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Title
- Flow cytometry studies on the populations and immune parameters of the hemocytes of the Suminoe oyster, Crassostrea ariakensis
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Author(s)
- Donaghy, Ludovic; Kim, Bong-Kyu; Hong, Hyun-Ki; Park, Heung-Sik; Choi, Kwang-Sik
- KIOST Author(s)
- Park, Heung Sik(박흥식)
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Alternative Author(s)
- 박흥식
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Publication Year
- 2009-08
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Abstract
- The Suminoe oyster Crassostrea ariakensis has been attempted to be introduced in the Chesapeake Bay, USA, as an alternative to the eastern oyster Crassostrea virginica. Commercial production of Suminoe oysters is currently restricted due to the incomplete understanding of their biological, physiological and immunological nature. Accordingly, understanding immune system of C. ariakensis is crucial to prevent disease associated mortality and subsequent management of the Suminoe oyster. We investigated immunological activities and morphology of hemocytes of the Suminoe oyster using flow cytometry and light microscopy. Three types of hemocytes were identified in the hemolymph including hyalinocyte, granulocyte and blast-like cells. Hyalinocytes were the largest cells and the most abundant, while granulocytes were intermediate-size cell containing numerous granules in the cytoplasm. Blast-like cells were the smallest and least numerous. Flow cytometry revealed that the granulocytes are most active in the cell phagocytosis and spontaneous reactive oxygen species (ROS) production. The hyalinocytes also showed a certain level of the phagocytosis and oxidative activity but in a lesser extent than the granulocytes. In contrasts the blast-like cells did not show any phagocytosis or oxidative activity. The flow cytometry used in this study confirmed that as observed from other marine bivalves, the granulocytes are the main hemocytes involved in the cellular defence in the Suminoe oyster. (C) 2009 Elsevier Ltd. All rights reserved.
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ISSN
- 1050-4648
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4277
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DOI
- 10.1016/j.fsi.2009.05.010
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Bibliographic Citation
- FISH & SHELLFISH IMMUNOLOGY, v.27, no.2, pp.296 - 301, 2009
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Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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Subject
- EUROPEAN FLAT OYSTER; MERCENARIA-MERCENARIA HEMOCYTES; DECUSSATUS MOLLUSCA-BIVALVIA; HAPLOSPORIDIUM-NELSONI MSX; CLAM TAPES-PHILIPPINARUM; RUDITAPES-PHILIPPINARUM; CHESAPEAKE BAY; OSTREA-EDULIS; MANILA CLAM; REACTIVE OXYGEN
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Keywords
- Crassostrea ariakensis; Suminoe oyster; Flow cytometry; Granulocytes; Hyalinocytes; Blast-like cells; Phagocytosis; Reactive oxygen species
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Type
- Article
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Language
- English
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Document Type
- Article
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