Heterologous Microarray Hybridization Used for Differential Gene Expression Profiling in Benzo[a]pyrene-exposed Marine Medaka SCIE KCI OTHER

Title
Heterologous Microarray Hybridization Used for Differential Gene Expression Profiling in Benzo[a]pyrene-exposed Marine Medaka
Author(s)
Woo, Seonock; Won, Hyokyoung; Jeon, Hye-young; Kim, Bora; Lee, Taek-Kyun; Park, Hong-Seog; Yum, Seungshic
KIOST Author(s)
Lee, Taek Kyun(이택견)Yum, Seungshic(염승식)
Publication Year
2009-12-31
Abstract
Differential gene expression profiling was performed in the hepatic tissue of marine medaka. fish (Oryzias javanicus) after exposure to benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), by heterologous hybridization using a medaka cDNA microarray. Thirty-eight differentially expressed candidate genes, of which 23 were induced and 15 repressed (P<0.01), were identified and found to be associated with cell cycle, development, endocrine/reproduction, immune, metabolism, nucleic acid/protein binding, signal transduction, or non-categorized. The presumptive physiological changes induced by BaP exposure were identified after considering the biological function of each gene candidate. The results obtained in this study will allow future studies to assess the molecular mechanisms of BaP toxicity and the development of a systems biology approach to the stress biology of organic chemicals.
ISSN
1738-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4230
Bibliographic Citation
MOLECULAR & CELLULAR TOXICOLOGY, v.5, no.4, pp.283 - 290, 2009
Publisher
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
Subject
POLYCYCLIC AROMATIC-HYDROCARBONS; MESSENGER-RNA EXPRESSION; ENVIRONMENTAL GENOMICS; SQUAMOUS CARCINOMA; ORYZIAS-JAVANICUS; ESTROGEN-RECEPTOR; OXIDATIVE STRESS; FISH; PROTEIN; INDUCTION
Keywords
Marine medaka; Oryzias javanicus; Heterologous hybridization; Microarray benzo[a]pyrene (BaP); Differential gene expression profile
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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