Anti-Inflammatory Effect of Turbo cornutus Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study
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Title
- Anti-Inflammatory Effect of Turbo cornutus Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study
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Author(s)
- Kim, Eun A; Kang, Na Lae; Kim, Jun Seong; Yang, Hye-Won; Ahn, Ginnae; Heo, Soo Jin
- KIOST Author(s)
- Kim, Eun A(김은아); Kang, Na Lae(강나래); Kim, Jun Seong(김준성); Heo, Soo Jin(허수진)
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Alternative Author(s)
- 김은아; 강나래; 김준성; 허수진
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Publication Year
- 2022-02
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Abstract
- Turbo cornutus, the horned turban sea snail, is found along the intertidal and basaltic shorelines and is an important fishery resource of Jeju Island. In this study, we performed a preliminary study on anti-inflammatory effect of 70% ethanol extract obtained from T. cornutus viscera (TVE) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells in vitro and zebrafish embryos in vivo. TVE reduced the production of LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE2) without any toxic effects. TVE also decreased the protein expression of LPS-induced inducible NO synthase and cyclooxygenase-2 and suppressed the production of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6, and IL-1β. Furthermore, mechanistic studies indicated that TVE suppressed c-Jun N-terminal kinase phosphorylation and nuclear factor-kB activation. In zebrafish embryos, TVE did not show developmental toxicity based on the survival rate and cell death findings. In LPS-stimulated zebrafish embryos, TVE suppressed NO production and cell death. In conclusion, the result from this preliminary study showed TVE has a potential anti-inflammatory property that can be exploited as a functional food ingredient. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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ISSN
- 2304-8158
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/42280
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DOI
- 10.3390/foods11030364
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Bibliographic Citation
- Foods, v.11, no.3, 2022
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Publisher
- MDPI
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Keywords
- Anti-inflammatory; RAW264.7 cells; Turbo cornutus; Viscera; Zebrafish
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Type
- Article
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Language
- English
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Document Type
- Article
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