Identifying Potential Antioxidant Properties from the Viscera of Sea Snails (Turbo cornutus)
SCIE
SCOPUS
Cited 10 time in
WEB OF SCIENCE
Cited 12 time in
Scopus
-
Title
- Identifying Potential Antioxidant Properties from the Viscera of Sea Snails (Turbo cornutus)
-
Author(s)
- Kang, Na Lae; Kim, Eun A; Kim, Jun Seong; Lee, Seung-Hong; Heo, Soo Jin
- KIOST Author(s)
- Kang, Na Lae(강나래); Kim, Eun A(김은아); Kim, Jun Seong(김준성); Heo, Soo Jin(허수진)
-
Alternative Author(s)
- 강나래; 김은아; 김준성; 허수진
-
Publication Year
- 2021-10
-
Abstract
- Turbo cornutus, the horned turban sea snail, is found along the intertidal and basaltic shorelines of Jeju Island, Korea. T. cornutus feeds on seaweeds (e.g., Undaria sp., and Ecklonia sp.) composed of diverse antioxidants. This study identified potential antioxidant properties from T. cornutus viscera tissues. Diverse extracts were evaluated for their hydrogen peroxide (H2O2) scavenging activities. T. cornutus viscera protamex-assisted extracts (TVP) were purified by gel filtration chromatography (GFC), and potential antioxidant properties were analyzed for their amino acid sequences and its peroxidase inhibition effects by in silico molecular docking and in vitro analysis. According to the results, T. cornutus viscera tissues are composed of many protein contents with each over 50%. Among the extracts, TVP possessed the highest H2O2 scavenging activity. In addition, TVP-GFC-3 significantly decreased intracellular reactive oxygen species (ROS) levels and increased cell viability in H2O2-treated HepG2 cells without cytotoxicity. TVP-GFC-3 comprises nine low molecular bioactive peptides (ELR, VGPQ, TDY, ALPHA, PAH, VDY, WSDK, VFSP, and FAPQY). Notably, the peptides dock to the active site of the myeloperoxidase (MPO), especially TDY and FAPQY showed the MPO inhibition effects with IC50 values of 646.0 & PLUSMN; 45.0 mu M and 57.1 & PLUSMN; 17.7 mu M, respectively. Altogether, our findings demonstrated that T. cornutus viscera have potential antioxidant properties that can be used as high value-added ingredients.
-
ISSN
- 1660-3397
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/42118
-
DOI
- 10.3390/md19100567
-
Bibliographic Citation
- MARINE DRUGS, v.19, no.10, 2021
-
Publisher
- MDPI
-
Keywords
- Turbo cornutus; viscera; antioxidant; hydrogen peroxide; bioactive peptide
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.