Enhanced H2 production by deletion of the Tfx family DNA-binding protein in the hyperthermophilic archaeon Thermococcus onnurineus NA1 SCIE SCOPUS

DC Field Value Language
dc.contributor.author Lee, Seong Hyuk -
dc.contributor.author Lee, Sung Mok -
dc.contributor.author Kang, Sung Gyun -
dc.contributor.author Lee, Hyun Sook -
dc.date.accessioned 2022-01-19T10:33:26Z -
dc.date.available 2022-01-19T10:33:26Z -
dc.date.created 2021-09-13 -
dc.date.issued 2021-10-14 -
dc.identifier.issn 0360-3199 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/42115 -
dc.description.abstract A gene encoding an archaeal transcription regulator (TON_1525) homologous to the Tfx family DNA-binding protein has been identified to affect gene expression of carbon monoxide metabolism in the hyperthermophilic archaeon Thermococcus onnurineus NA1. To broadly understand gene regulation by the TON_1525 gene, a gene deletion mutant (Δ1525) was constructed and transcriptome changes were investigated. A total of 351 genes were found to be differentially expressed in the Δ1525 mutant. The expression levels of the hydrogenase genes were significantly changed; mbh (membrane-bound hydrogenase) and mch (membrane-bound carbon monoxide-dependent hydrogenase) genes were increased, and mfh2 (membrane-bound formate-dependent hydrogenase) and soluble hydrogenase genes were decreased. The Δ1525 mutant was cultured in a medium supplemented with maltodextrin or carbon monoxide to investigate whether changes in gene expression lead to physiological changes. The maximum H2 production rates of the Δ1525 mutant were significantly enhanced on both substrates compared to the wild-type strain. These results indicate that the TON_1525 gene can affect the expression of various genes including mbh and mch, consequently regulating the metabolism of T. onnurineus NA1. This study expands our understanding of the functional role of the Tfx family DNA-binding proteins. © 2021 -
dc.description.uri 1 -
dc.language English -
dc.publisher Elsevier Ltd -
dc.title Enhanced H2 production by deletion of the Tfx family DNA-binding protein in the hyperthermophilic archaeon Thermococcus onnurineus NA1 -
dc.type Article -
dc.citation.endPage 35197 -
dc.citation.startPage 35189 -
dc.citation.title International Journal of Hydrogen Energy -
dc.citation.volume 46 -
dc.citation.number 71 -
dc.contributor.alternativeName 이성혁 -
dc.contributor.alternativeName 이성목 -
dc.contributor.alternativeName 강성균 -
dc.contributor.alternativeName 이현숙 -
dc.identifier.bibliographicCitation International Journal of Hydrogen Energy, v.46, no.71, pp.35189 - 35197 -
dc.identifier.doi 10.1016/j.ijhydene.2021.08.075 -
dc.identifier.scopusid 2-s2.0-85113957601 -
dc.identifier.wosid 000709709500012 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.description.isOpenAccess N -
dc.subject.keywordPlus Carbon monoxide -
dc.subject.keywordPlus Gene encoding -
dc.subject.keywordPlus Metabolism -
dc.subject.keywordPlus Microorganisms -
dc.subject.keywordPlus Proteins -
dc.subject.keywordPlus Transcription -
dc.subject.keywordPlus Archaeal transcription -
dc.subject.keywordPlus DNA-binding protein -
dc.subject.keywordPlus Genes encoding -
dc.subject.keywordPlus Genes expression -
dc.subject.keywordPlus H$-2$/ production -
dc.subject.keywordPlus Hydrogenases -
dc.subject.keywordPlus Hyperthermophilic archaeon -
dc.subject.keywordPlus Membrane-bound -
dc.subject.keywordPlus Tfx family DNA-binding protein -
dc.subject.keywordPlus Thermococcu onnurineu NA1 -
dc.subject.keywordPlus DNA -
dc.subject.keywordPlus Substrates -
dc.subject.keywordAuthor H2 production -
dc.subject.keywordAuthor Hydrogenase -
dc.subject.keywordAuthor Tfx family DNA-Binding protein -
dc.subject.keywordAuthor Thermococcus onnurineus NA1 -
dc.relation.journalWebOfScienceCategory Chemistry, Physical -
dc.relation.journalWebOfScienceCategory Electrochemistry -
dc.relation.journalWebOfScienceCategory Energy & Fuels -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Chemistry -
dc.relation.journalResearchArea Electrochemistry -
dc.relation.journalResearchArea Energy & Fuels -
Appears in Collections:
Marine Resources Research Division > Marine Biotechnology Research Center > 1. Journal Articles
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